研究证实M2型肿瘤相关巨噬细胞（TAM）分泌一些细胞因子及趋化因子而促进肿瘤细胞的增殖、侵袭及转移；且TAM侵袭增加与乳腺癌的耐药、远处转移及预后差密切相关。在乳腺癌进展的不同时期及治疗过程中TAM聚集的位置及数量会发生改变，如肿瘤快速进展时更多聚集在邻近血管生成部位且数量增多；治疗有效则数量减少。因此，通过显示出TAM能否有助于临床评估乳腺癌的进展情况值得深入研究。甘露醇受体（CD206 ）为仅表达于TAM的特异性受体，CD206有望成为TAM显影靶点。本研究拟在我们前期制备含-COOH末端纳米靶向脂质微泡的研究基础上在成膜材料中加入PH敏感脂质材料及金纳米棒，以液态氟碳（PFP）为成像气体；通过碳化二亚胺的方法将CD206连接至造影剂表面；金纳米棒能进行光热转换辅助PFP液气相变。期望利用其PH响应性及主动靶向性显示乳腺癌中TAM，为临床评估乳腺癌进展情况提供科学依据。

The steps of macrophage trafficking to the tumor siteis guided by the presence of chemotactic and chemostatic molecules leading to the mobilization in different regions of the breast tumor.Tumor-associated macrophages (TAMs) are a set of macrophages residing in the tumor microenvironment, which are recruited to the tumor site from the bone marrow in response to signals released by both tumor and stromal cells. M2-polarzied TAMs promote tumor cell migration and invasion by secreting cytokines and chemokines. Increasing evidence from clinical and preclinical studies has indicated that TAMs promote tumor cell invasion, proliferation, survival, and metastasis to local and distant sites.Several clinical studies have demonstrated that increased macrophage infiltration into tumors confers metastatic potential and poor prognosis in breast cancer. M2-like macrophage populations are found at the invasive edge of breast tumors. In many cancers, including breast cancer, the increased presence of TAMs is associated with increased aggressiveness of tumors and decreased patient’s survival.Macrophage mannose receptor (MMR; CD206) is not expressed in M1 macrophages; therefore, CD206 serves as a useful marker to distinguish M2 from M1 macrophages.This study is based on our previous research on the preparation of COOH ended long circulating nano lipid microbubbles. Furthermore, we try to prepare nanobubble which contain COOH group.PFP is contained in the Nanobubble, whicn can change into gas . Gold nanorods were also added in order to enhance ability of PFP phase- transition.By observing the proportion of materials, ultrasonic oscillation of time and energy on the diameter, Zeta potential, stability of the nanomicelles, a best proposal to produce the gold nanorods loading nanobubbles would be found. By carbodiimide method(EDC/NHS), CD206 is connected to the COOH end of the nanobubbles. Then testing the targeting effect in vitro and in vivo. It has important significance to evaluate the progesss of the breast cancer