动物性食品中多组分兽药残留并存的问题普遍存在，传统兽药残留检测方法主要基于抗原-抗体的特异性免疫反应，虽然已广泛使用，但难以实现多检测靶标、高通量、定量检测。为突破传统免疫分析技术瓶颈，本项目拟搭建碟式离心微流控分析平台：（I）通过3D建模和激光雕刻，设计和加工碟式离心微流控芯片，研究芯片结构与孔道表面处理对微液滴运动的影响；（II）采用伺服电机作为芯片的离心力驱动源、制冷电荷耦合器件作为光学信号捕获装置，搭建碟式离心微流控技术平台，研究微液滴离心驱动方式和光学信号定量方式；（III）选取牛奶中6种典型兽药（β-内酰胺、磺胺、氯霉素、喹诺酮、大环内脂、氨基糖苷），采用竞争免疫反应模式，建立碟式离心微流控检测新方法。本研究可为动物性食品中多组分兽药残留的高通量、定量检测提供新的研究思路和技术手段，对保障动物性食品安全具有重要意义。

The multi veterinary drug residues in animal food is a critical issue. Traditional analysis methods are mainly based on the specific immunoreaction of antigen-antibody and have been widely used. However, it is difficult to achieve multi-targets, high-throughput, and quantitative detection. In order to break through the bottlenecks of traditional immunoassay, this project intends to build a centrifugal microfluidic immunoassay platform: (I) Centrifugal microfluidic chip will be designed and fabricated by 3D modeling and laser engraving. We will study the influence of chip structure and surface modification on micro-liquid handling; (II) Servo motor is used as the centrifugal force driving source and the cooled charge-coupled device is used as the optical signal capture device. The centrifugal driving method and the quantitative approach in micro-liquid will be studied; (III) 6 typical veterinary drug residues (Beta-lactam, sulfonamide, chloramphenicol, quinolone, macrolipids, and aminoglycosides) were selected as target to establish a centrifugal microfluidic-based competitive immunoassay. This study can provide new ideas for high-throughput and quantitative detection of multi veterinary drug residues in animal food, which is of great significance for ensuring the animal food safety.