黄瓜花叶病毒（CMV）携带卫星RNA（satRNA）是一种普遍的生物学现象。satRNA的侵染通常会降低CMV的积累，从而减轻病毒的症状。但是，satRNA致弱的分子机制仍知之甚少。前期研究发现：satRNA T1分离物（sat-T1）选择性地抑制CMV RNA1和RNA2的复制。本项目以此作为出发点，将通过选择性的2’羟基酰基化和引物延伸（SHAPE）的方法，结合生物信息学手段和突变体的生物学实验，确定sat-T1的RNA结构。在此基础上，寻找和测定sat-T1抑制CMV RNA1和RNA2复制的致弱功能域。最后从致弱功能域RNA与CMV RNA1和RNA2分子间的互作、致弱功能域RNA与寄主蛋白因子的互作两方面进行分析，阐明sat-T1抑制CMV RNA1和RNA2复制的作用机理，揭示sat-T1减轻病毒症状的新机制。该项目的顺利实施有望为开发和建立抗CMV新方法提供新的靶点和策略。

It is a common biological phenomenon that cucumber mosaic virus (CMV) carries a satellite RNA (satRNA). Infection with satRNA usually reduces CMV accumulation, thus attenuates CMV-induced symptoms. However, the intrinsic mechanism is largely unknown. Our previous finding shows that the isolate T1 of satRNA selectively inhibits replication of RNAs 1 and 2 of CMV. On the basis of this finding, this proposal will determine the RNA structure of sat-T1 by selectively 2’-hydroxyl acylation and primer extension (SHAPE), combined with bioinformatic prediction and biological assays of mutants. Subsequently, an effort will be made to seek and determine benign domains by which sat-T1 inhibits replication of CMV RNA1 and RNA2. Finally, functional basis by which sat-T1 inhibits replication of CMV RNA1 and RNA2 would be explored by analyzing interaction of the benign domain of sat-T1 with CMV RNA1 and RNA2 or with host protein factors, which would dissect a novel mechanism of attenuating CMV-induced symptom expression by sat-T1. If working well with the project, it would provide a new target and strategy for development and establishment of novel anti-CMV methods.