草鱼×赤眼鳟杂交子一代抗草鱼出血病分子机理研究

草鱼是我国第一大淡水养殖品种，草鱼出血病长期制约产业的健康发展；本项目着眼草鱼种质资源改良，探索从根本上解决草鱼出血病的治本途径。以申请者2011年成功获得草鱼×赤眼鳟远缘杂交F1群体为研究材料，在初步比较杂交F1、草鱼、赤眼鳟对草鱼出血病病毒免疫特性差异基础上，采用文库构建、生物信息学分析、克隆与表达等技术，从生理生化、基因表达和转录组测序等方面综合分析，确定杂交F1抗草鱼出血病相关的主要信号通路和关键基因，比较关键基因介导免疫信号通路的表达调控，探究抗病分子机理，旨在挖掘抗草鱼出血病关键基因、筛选抗病草鱼新材料。这不仅可丰富鱼类抗性育种理论，而且对抗草鱼出血病新品种（系）选育实践具有重要的指导价值。

Grass carp (Ctenopharyngodon idellus)is the first aquacultural production of freshwater fish species in China. However, the healthy development of aquaculture has been restricted by the hemorrhage disease. Focusing on improving the germplasm resources of the grass carp, this project will explore a fundamental way to solve the hemorrhage disease of grass carp. The study material is the F1 population, which was got by the distant hybridization between grass carp and barbel chub (Spualiobarbus Curriculus) in 2011. In order to find out the main signaling pathways and the key genes related to disease resistance of grass carp, the modern molecular biotechnology, such as library construction, gene cloning, protein expression, bioinformatics analysis, will be used. By comprehensive analysis of those results of physiological and biochemical tests, gene expression and transcriptome sequencing, the function and molecular mechanism on disease resistance of the pathways and its key genes would be able to find out. In this project, the new disease resistance material from distant hybridization will be studied to excavate the key genetic resources of hemorrhage disease and its disease resistance mechanism. Once it is succeeded, it can not only enrich the fish resistance breeding theory, but also provide an important guiding value towards breeding practice in grass carp hemorrhage disease resistance.