草鱼出血病严重制约草鱼产业健康发展，选育高抗性草鱼成为行业学界热点。本团队前期研究发现母源免疫草鱼子代的GCRV抗性显著增强且TLR受体激活为草鱼免疫保护启动的重要途径，母源免疫处理草鱼受精卵的TLR13/20是仅鉴定出的表达显著上调模式识别受体。本项目拟以母源免疫草鱼亲本与子代为材料，基于过表达、RNA干扰、免疫共沉淀、双荧光素酶报告实验和启动子表达调控分析等技术进一步深入研究（1）母源免疫处理引发草鱼受精卵TLR13/20表达上调的分子机制；（2）TLR13/20表达的母本至子代传递效率及抗GCRV免疫功能。项目研究旨在明确TLR13/20在母源免疫增强草鱼子代抗性中的地位及功能机制，为抗病草鱼新材料创制提供分子依据及新思路。

The healthy development of grass carp (Ctenopharyngodon idellus) industry is severely restricted by grass carp hemorrhage disease. The selective breeding of C. idellus with high resistance is research focus of industry and academia. Our previous research found that maternal immunization could significantly enhance the progeny resistance to grass carp reovirus (GCRV) and the activation of Toll-like receptor (TLR) is an important pathway for the achievement of immune protection by vaccination, and TLR13/20 are the only two identified pattern recognition receptors those significantly up-regulated in eggs of maternal immunized C. idellus. This project is intended to further investigate: (1) molecular mechanism of the effect of maternal immunization on up-regulation of TLR13/20 in eggs of C. idellus; (2) expression transmission efficiency of TLR13/20 from female parent to progeny and their GCRV resistance functions; by applying the over-expression, RNA interference, co-Immunoprecipitation, dual-luciferase reporter assay and promoter expression regulation analysis. This study aims to figure out the roles and functional mechanism of TLR13/20 in resistance enhancement of progeny by maternal immunization, and to provide molecular information and new thoughts for the breeding of C. idellus with viral resistance.