诱导卵巢癌干细胞（OCSC）凋亡为卵巢癌治疗提供了有效途径，但目前尚缺乏安全、高效、可控的基因递送系统。鉴于OCSC高表达Lin28和Oct4，本研究在前期研究基础上，以壳聚糖为泡膜材料，利用PEI-溴化十六烷基三甲胺、适配子和RGD、USPIO和量子点修饰壳聚糖，制备具有长循环、高负载率、磁响应、双靶向（CD133适配子和RGD多肽）及多模态分子成像能力的携miRNA多功能纳米气泡（MN），用于OCSC基因治疗和监测。该载基因气泡在声辐射力（ARF）和磁场的作用下靶向聚集于肿瘤，并通过其表面的配体选择性与OCSC结合，ARF可促使MN破坏，促进miRNA进入胞内并诱导OCSC凋亡，达到多重主动靶向和智能调控的目的。同时，运用多模态分子成像对OCSC治疗过程进行可视化观察和早期疗效监测，以达到有效治疗与调控。本研究将纳米基因靶向递送系统和miRNA技术相结合，为肿瘤基因治疗提供新方法。

Inducing apoptosis of ovarian cancer stem cells (OCSC) by miRNA is of great value to the successful eradication of cancer, but there is still lack of a safe, efficient and controllable system to delivery therapeutic genes to OCSC. On the basis that Lin28 and Oct4 genes are overexpressed in OCSC, this study is to modify chitosan with PEI and cetyltrimethylammonium bromide, aptamer, RGD peptide, USPIO and quantum dot to prepare a multifunctional nanobubble (MN) which can carry the miRNA expression vector targeting lin28 and Oct4 to realize target therapy of ovarian cancer. The main strengths of MN include long-circulation time, low toxicity, high gene load capacity, magnetic response, double targeting and it can be used for multimodal imaging. With the exposure of acoustic radiation force (ARF) and magnetic field, MN can accumulate in the tumor and can bind to CSC selectively with CD133 aptamer and RGD peptide. Furthermore, the exposure of ARF can break MN and liberate genes locally while increasing cellular uptake of them. Thus, it combines multiple initiactive targeted delivery, and ultrasound smart control of gene release to inhibit OCSC with miRNA. Additionally, via multimodal molecular imaging, this study conducts quantitative and real time observations of OCSC treatment to achieve effective gene therapy and regulate target therapy of OCSC. Our study aims to combine nanobubble delivery system and miRNA technology to cure ovarian cancer, which will provide a novel strategy for cancer therapy.