课题基金基金详情
蓝藻NDH-1-PSI在胁迫条件下的形成机制及其生理功能分析
结题报告
批准号:
31700205
项目类别:
青年科学基金项目
资助金额:
25.0 万元
负责人:
高复旦
依托单位:
学科分类:
C0203.植物光合与固氮
结题年份:
2020
批准年份:
2017
项目状态:
已结题
项目参与者:
赵娇红、冉兆星、肖婷婷、潘秀、石楠、郑梅
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中文摘要
蓝藻NDH-1能通过CpcG2-PBS与PSI形成超分子复合物NDH-1-PSI,并专一地参与围绕PSI的环式电子传递。然而,NDH-1-PSI在胁迫条件下的形成机制及其生理功能均尚不清楚。最近,我们的初步结果指出光质(绿光和红光)能够改变CpcG2-PBS和NDH-1-PSI的数量但不影响NDH-1和PSI的数量。因此,本项目将首先证实光质对NDH-1-PSI及其组分的动态关系,进而揭示出NDH-1-PSI在光质胁迫条件下的形成机制及其生理功能。然后,通过分子和生理生化等手段分析其他胁迫条件下NDH-1-PSI及其组分的动态关系,找出影响NDH-1-PSI形成的关键组分,进而揭示出NDH-1-PSI在其他胁迫条件下的形成机制及其生理功能。这些研究结果必将增进人们对蓝藻NDH-1-PSI在胁迫条件下重要角色的理解,也为研究高等植物NDH-1-PSI的形成机制和生理功能提供重要的理论参考。
英文摘要
Cyanobacterial NDH-1 can interact with PSI to form an NDH-1-PSI supercomplex via a light-harvesting antenna CpcG2-phycobilisome (PBS) and the supercomplex is exclusively involved in cyclic electron transport around PSI (NDH-CET). However, the forming mechanism and physiological function of NDH-1-PSI under stress conditions remains elusive. Recently, we found that quality of light including green light and red light altered the amounts of CpcG2-PBS but did not affect the protein levels of NDH-1 and PSI. Thus, we will first verify the dynamic relationship regarding the effects of light quality on NDH-1-PSI and its members, NDH-1, CpcG2-PBS and PSI; consequently, we will reveal the forming mechanism of NDH-1-PSI under stress conditions of light quality. Further, we will clarify the physiological function of NDH-1-PSI under conditions of different qualities of light. Next, we will analyze the dynamic relationship regarding the effects of other stresses on NDH-1-PSI and its members, find out the key member for the formation of NDH-1-PSI, and reveal the forming mechanism of NDH-1-PSI. Finally, we will clarify the physiological function of NDH-1-PSI under conditions of other stresses using strategies of molecular biology, plant physiology and etc. These results will certainly increase our understanding the important role of cyanobacterial NDH-1-PSI supercomplex under stress conditions and provide an important the theory reference for understanding the forming mechanism and physiological function of NDH-1-PSI in higher plants.
蓝藻NDH-1能通过CpcG2-PBS与PSI形成超分子复合物NDH-1-PSI,并专一地参与围绕PSI的环式电子传递(NDH-CET)。然而,NDH-1-PSI在胁迫条件下的形成机制及其生理功能均尚不清楚。我们通过蓝绿温和凝胶电泳(BN-PAGE)和蛋白免疫印迹等手段,在绿光和红光(对照:红光,为绿光的互补光)等生长条件下,分析NDH-1-PSI,以及它的组分NDH-1,CpcG2-PBS和PSI的亚基数量变化情况,发现绿光能诱导上调NDH-1-PSI及其组分CpcG2-PBS的亚基数量,但不影响该超分子复合体的其他组分NDH-1和PSI的亚基数量。因此,我们认为绿光通过增加CpcG2-PBS的量,增强了NDH-1与PSI的连接,使得NDH-1-PSI的数量增加,从而使NDH-1和PSI活性上调,加快了NDH-CET。然后,通过分子和生理生化等手段分析低碳等其他胁迫条件下NDH-1-PSI及其组分的动态关系,发现低碳胁迫下通过上调NDH-1的量来增加NDH-1-PSI的量,从而增加NDH-1活性。这些研究结果增进人们对蓝藻NDH-1-PSI在胁迫条件下重要角色的理解,也为研究高等植物NDH-1-PSI的形成机制和生理功能提供了重要的理论参考。
期刊论文列表
专著列表
科研奖励列表
会议论文列表
专利列表
Effect of green light on the amount and activity of NDH-1-PSI supercomplex in Synechocystis sp strain PCC 6803
绿光对集胞藻PCC 6803菌株NDH-1-PSI超复合物数量和活性的影响
DOI:10.1007/s11099-018-0790-z
发表时间:2018
期刊:Photosynthetica
影响因子:2.7
作者:Gao Fudan;Ogawa Teruo;Ma Weimin
通讯作者:Ma Weimin
DOI:10.1093/pcp/pcz045
发表时间:2019-06-01
期刊:PLANT AND CELL PHYSIOLOGY
影响因子:4.9
作者:Ran, Zhaoxing;Zhao, Jiaohong;Ma, Weimin
通讯作者:Ma, Weimin
DOI:10.3389/fpls.2017.02183
发表时间:2017
期刊:Frontiers in plant science
影响因子:5.6
作者:Zhao J;Gao F;Fan DY;Chow WS;Ma W
通讯作者:Ma W
DOI:--
发表时间:2020
期刊:Frontiers in Microbiology
影响因子:5.2
作者:Fudan Gao
通讯作者:Fudan Gao
国内基金
海外基金