汗腺再生研究具有较高科研价值和临床意义。研究表明，特异性微环境调控汗腺解剖结构的发育；而血管化水平影响汗腺生理功能。我们发现，二甲基精氨酸二甲胺水解酶-1（DDAH-1）在汗腺发育微环境中高表达，且DDAH-1在汗腺细胞和表皮细胞的表达量有显著性差异，提示DDAH-1是汗腺微环境的特异蛋白。我们拟证明：DDAH-1参与“微环境调控”间充质干细胞（MSCs）向汗腺细胞定向分化。首先，验证DDAH-1在汗腺微环境的差异性表达。然后，分别高表达（质粒）或沉默（siRNA）DDAH-1基因，检测MSCs向汗腺分化情况，并在裸鼠活体实现汗腺再生。最后，筛选确定DDAH-1下游信号分子。本研究拟利用DDAH-1既是微环境特异蛋白又可促血管化的“双重调控作用”，实现汗腺解剖结构和生理功能的同步再生，阐明相关机制，为临床转化提供借鉴。

The regeneration study about sweat glands is of high scientific value and clinical significance. Studies have shown that specific microenvironment regulates the development of sweat gland anatomical structures, and vascularization level affects the physiological function of sweat glands. We found that dimethyl arginine dimethylamine hydrolase-1 (DDAH-1) was highly expressed in sweat gland microenvironments and the expression of DDAH-1 was significantly different between sweat gland cells and epidermal cells, suggesting that DDAH-1 was a specific protein in sweat gland microenvironments. We intend to demonstrate that DDAH-1 is involved in the "microenvironment regulation" during mesenchymal stem cells (MSCs) differentiate into sweat gland cells. Firstly, the differential expression of DDAH-1 in sweat gland microenvironments will be verified. Secondly, the DDAH-1 genes will be highly expressed (plasmid) or be silenced (siRNA) in order to detect the differentiation of MSCs into sweat glands and further realize the regeneration of sweat glands in living nude mice. Finally, the downstream signal molecules of DDAH-1 will be screened and verified. In this study, we intend to achieve synchronous regeneration of the anatomical structure and physiological functions of sweat glands by using the "dual regulatory effect" of DDAH-1, which is both a microenvironment-specific protein and a vascularization promoter, so as to clarify the relevant mechanisms and provide reference for clinical transformation.