多能性干细胞是具有无限自我更新和多潜能分化能力的一类干细胞，是再生医学治疗中的重要细胞来源。维持干细胞的自我更新、干细胞高效定向分化是临床应用的基础，但到目前为止，干细胞的自我更新和分化的调控机制不清楚，研究手段有限，阻碍了干细胞的临床应用发展。而单倍体胚胎干细胞由于只有单等位基因，结合转座子系统将可能高效的进行大规模基因筛选；并且通过同源重组，可以用于快速高效的获得基因打靶的单倍体干细胞系。因此，我们将利用现有的单倍体胚胎干细胞，建立一个高效快速的筛选系统及研究基因功能的平台，对干细胞的自我更新和分化的调控机制开展研究，从而为干细胞的临床应用奠定基础。

The pluripotent stem cells can self-renew and differentiate into various cell types, have promising future in regeneration medicine. However, as the molecular mechanism of self-renewal and differentiation of pluripotent stem cells is unclear, the maintenance of pluripotent stem cells and direct differentiation in defined culture system is difficult yet, which hinder the clinical application. The haploid stem cells which contain only one allelic gene, can utilize in genetic screening when combine with piggyback transposon mutation system. The haploid stem cells also can be used to generate gene knockout cell lines by homologous recombination quickly. With the haploid stem cells, we can screen putative genes and explore the molecular mechanism of promote self-renewal quickly, which may promote the clinical application in future.