转录因子调控靶基因表达参与多种生理过程。近年来转录因子的鉴定、调控靶基因表达机制的研究逐渐成为昆虫学研究的热点，但其介导农业害虫抗药性的研究仍较缺乏。柑桔全爪螨作为世界性害螨可为害多种寄主植物，化学防治导致的抗性给安全生产带来重大隐患。本项目拟以柑桔全爪螨为研究对象，鉴定转录因子CncC，分析在阿维菌素抗、敏品系中表达差异；利用BIAcore技术分析CncC重组蛋白与Cyp392A24间结合活性和动力学；测定沉默CncC对Cyp392A24表达量及阿维菌素杀螨活性的影响；测定沉默Cyp392A24对阿维菌素敏感性变化；测定Cyp392A24重组蛋白活性及动力学参数，分析其对阿维菌素的离体代谢能力。最终将揭示转录因子CncC调控Cyp392A24过表达介导柑桔全爪螨对阿维菌素抗性机制。研究结果将为柑桔全爪螨抗性治理及开发新的抗性检测方法，制定有效的柑桔全爪螨防控策略奠定理论基础。

Transcription factor play a critical role in many biological processes by regulating target gene expression. Recently, identification, regulation mechanism and functional analyses of insect transcription factor has attracted more attention in field of insect research, whereas, research on insecticide resistance mediated by transcription factor in agricultural pests are less. Chemical pesticides spraying as main strategy for control the citrus red mite, Panonychus citri, one of the most destructive mites of fruit and vegetables, resulted in insecticides resistance which is severely threatening the security of its production. In present study, transcription factor CncC will be identified and characterized based on the relative information in Tetranychus urticae and Tetranychus cinnabarinus. The gene expression level in abamectin susceptible and resistant strains will be investigated by qRT-PCR. The binding activity and kinetics of CncC recombination protein in Sf9 with Cyp392A24 will be tested by BIAcore technology. The influence on gene expression of Cyp392A24 and acaricidal activity of abamectin after silencing CncC will be conducted based on established high efficiency silencing system. In the meanwhile, the susceptibility to abamectin will be analyzed after Cyp392A24 silencing of P. citri, and the activity and kinetics of recombination protein expressed in E. coli, then the metabolic capability and metabolites of recombination protein against abamectin will be conducted by HPLC and HPLC-MS technology. Above all, eventually elucidate molecular mechanism of abamectin resistance mediated by overexpression of Cyp392A24 regulated by transcription factor CncC in P. citri. The research could provide theoretical basis for resistance management, development of novel method of resistance detection and effective control strategy.