协同性细胞迁移在发育、生理和疾病中非常普遍，但是目前还缺乏研究。果蝇卵巢里的边界细胞可作为模型系统研究协同性细胞迁移。我们已有的研究数据表明actin装配与Rab11介导的囊泡运输均参与调控了边界细胞迁移，且两者存在相互作用，但是这种相互作用的具体机制尚不清楚。本项目拟采用遗传学、细胞生物学、分子生物学和活体影像学多种方法，阐明F-actin和Rab11在边界细胞簇中的分布；通过改变Rab11或F-actin，观察F-actin或Rab11在边界细胞中的变化，以及对边界细胞迁移极性和信号传递的影响；探索联系F-actin与Rab11的中间因子。预计研究结果将深化对协同性迁移分子机制的认知，也将为肿瘤转移和其它细胞生命活动的机制研究提供参考。

Collective migration plays an important role in many physiological and pathological processes, but the mechanisms are unclear. Border cell migration in Drosophila ovary is an excellent model system to study collective cell migration. Our previous study revealed that actin dynamics and Rab11-positive vesicles both regulate border cell migration, and there is an interaction between F-actin and Rab11-positive vesicles. In this proposed study, we will use genetics, cell biology，molecular biology, and live image to study the localization of F-actin and Rab11 in border cell clusters, the connection between actin dynamics and Rab11-positive vesicles during border cell migration, and find the mediator between F-actin and Rab11. We anticipate that results from this proposed study will elucidate the mechanisms of collective cell migration, and provide new insights into tumor metastasis and other cellular processes.