我们前期研究表明，化香树果序醇提物(EPS)可诱导鼻咽癌细胞相互融合，胞内产生大量囊泡形成巨泡式死亡，而EPS在正常鼻咽细胞中无此作用，据此推测EPS有望用于鼻咽癌抗肿瘤治疗。网络药理学分析及验证发现EPS可作用于RAS/MAPK/AP-1信号通路进而调控下游基因，由此我们提出假说：EPS诱导鼻咽癌细胞巨泡式死亡的机制可能是通过调控RAS/MAPK/AP-1信号通路及其下游靶基因来实现。我们拟进一步以EPS处理的鼻咽癌细胞株CNE1和CNE2为研究对象，首先采用转录组、蛋白质组、染色质组、生物信息学等技术分析RAS/MAPK/AP-1信号通路下游靶基因、靶蛋白及其结合位点，其次用质粒转染、shRNA干扰和小分子抑制剂等方法进行基因功能分析，最后在鼻咽癌动物模型中验证EPS诱导巨泡式死亡以及抑制肿瘤细胞生长的作用机制。研究结果将为寻找治疗鼻咽癌新的药物提供重要线索，并拓展对巨泡式死亡的认识。

Our preliminary study showed that ethanol extract of infructescence of Platycarya.strobilacea Sieb.et Zucc. (EPS) is associated with the generation of cell fusion, perturbation of lysosomal vesicle transportation and the induction of methuosis in nasopharyngeal carcinoma (NPC) cell lines while this is not observed in the normal nasopharyngeal cell. Thus, we speculate that EPS may has anticancer effects. Network pharmacology analysis were conducted indicating that EPS mainly regulates the RAS/MAPK/AP-1 signaling pathway. So we hypothesis that the effects of EPS in NPC cells may be achieved through its regulation of the RAS/MAPK/AP-1 signaling pathway. Therefore this project is to further study the human NPC cell lines CNE1 and CNE2 which were treated with EPS. Firstly using RNA sequencing (RNA-seq), isobaric tags for relative and absolute quantitation (iTRAQ), Chromatin Immunoprecipitation-sequencing (ChIP-seq), bioinformatics and other technical methods, we will identify the potential targets in the RAS/MAPK/AP-1 signaling pathway. Secondly, the related gene function analysis will be investigated by plasmid transfection, shRNA interference and small molecule inhibitors technology. Finally, exam the treatment of EPS on NPC model in vivo. The results will provide important scientific basis for the anticancer effects of EPC in NPC cells and expand the understanding of methuosis.