Ethanol Extract of the Infructescence of Platycarya strobilacea Sieb. et Zucc. Induces Methuosis of Human Nasopharyngeal Carcinoma Cells
Ethanol Extract of the Infructescence of Platycarya strobilacea Sieb. et Zucc. Induces Methuosis of Human Nasopharyngeal Carcinoma Cells
复制标题
化香果序的乙醇提取物。
DOI：
10.1155/2020/2760979
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发表时间：
2020-04
影响因子：
--
通讯作者：
Fu Chaomei
中科院分区：
文献类型：
--
作者：
Liu Jinkun;Ying Min;Wu Bin;Fu Chaomei
对化香树的果序进行了研究。et Zucc. (PS)临床上用于治疗鼻炎和鼻窦炎。初步研究表明，紫茎泽兰果序乙醇提取物（EPS）具有明显的体外抗鼻咽癌作用。然而，EPS诱导的细胞死亡的机制尚不清楚。本研究旨在探讨EPS对鼻咽癌细胞的抑制作用及其机制。以CNE 1和CNE 2细胞为模型，观察EPS对鼻咽癌细胞的体外作用。用荧光显微镜、透射电镜和流式细胞仪观察EPS作用后细胞形态学的变化。通过网络药理学分析其潜在机制，并通过western blot分析进一步验证。EPS的抗肿瘤作用与CNE 1和CNE 2细胞融合和空泡的产生、溶酶体囊泡转运的干扰以及诱导凋亡有关。网络药理学和western blot结果表明，EPS对鼻咽癌细胞的作用可能是通过调节Ras原癌基因（RAS）/丝裂原活化蛋白激酶（MAPK）信号通路和转录因子c-Fos原癌基因（c-FOS）及其下游基因实现的。EPS诱导NPC细胞死亡的机制。其机制可能与转录因子c-FOS及其下游基因的调控有关。
The infructescence of Platycarya strobilacea Sieb. et Zucc. (PS) has been used in the treatment of rhinitis and sinusitis in clinical practice. Our preliminary study showed that an ethanol extract of the infructescence of PS (EPS) had significant antinasopharyngeal carcinoma (NPC) effects in vitro. However, the mechanism underlying the NPS cell death induced by EPS remains unclear. The aim of the present study was to investigate the inhibitory effects of EPS on NPC cells and to elucidate the underlying mechanism. The effects of EPS on NPC cells were investigated in CNE1 and CNE2 cells in vitro. In EPS-treated cells, the cell morphological changes were evaluated through fluorescence microscope, transmission electron microscopy, and flow cytometry. The underlying mechanism was analyzed via network pharmacology and further verified by western blot analysis. The anticancer effects of EPS were associated with the generation of CNE1 and CNE2 cell fusion and vacuoles, the perturbation of lysosomal vesicle transportation, and the induction of methuosis. The network pharmacology and western blot results indicated that the effect of EPS in NPC cells might be achieved via regulation of the Ras proto-oncogene (RAS)/mitogen-activated protein kinase (MAPK) signaling pathway and the transcription factor c-Fos proto-oncogene (c-FOS) and its downstream genes. EPS induces NPC cell death through methuosis. The mechanism might be related to regulation of the transcription factor c-FOS and its downstream genes.
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DOI：
10.1186/1478-811x-8-23
发表时间：
2010-09-07
期刊：
Cell communication and signaling : CCS
影响因子：
--
作者：
Parri M;Chiarugi P
通讯作者：
Chiarugi P
影响因子：
0.6
作者：
Liangliang Zhang;Yongmei Wang;Man Xu
通讯作者：
Liangliang Zhang;Yongmei Wang;Man Xu
影响因子：
7.5
作者：
Ridley AJ
通讯作者：
Ridley AJ
影响因子：
51.1
作者：
Tang, Xin-Ran;Li, Ying-Qin;Ma, Jun
通讯作者：
Ma, Jun
DOI：
--
发表时间：
2016
期刊：
Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials
影响因子：
--
作者：
Xiao-ni Jia;Jun-xian Wang;Yu-lin Cheng;Xu Zhi;Maohua Wang
通讯作者：
Xiao-ni Jia;Jun-xian Wang;Yu-lin Cheng;Xu Zhi;Maohua Wang