葡萄霜霉病是由霜霉菌引起的、全球范围内危害葡萄生产的一大卵菌病害。RxLR效应蛋白在卵菌与植物互作中发挥着重要作用。实验室前期的转录组分析发现了一个RxLR类效应因子，将其命名为RxLR31197。本研究首先克隆RxLR31197基因，以小鼠促凋亡蛋白Bax为诱导因子，分析其对Bax诱导的过敏性细胞死亡的抑制作用和诱导烟草细胞死亡的功能。同时对其关键识别位点及亚细胞定位进行分析，明确其毒性功能及其功能位点。然后以RxLR31197为诱饵，筛选葡萄霜霉菌诱导的中国野生复叶葡萄‘留坝-8’叶片酵母表达cDNA文库，获得与其互作的靶蛋白，并通过酵母回复实验、免疫共沉淀及双分子荧光互补进一步验证其互作。初步揭示寄主细胞抗病蛋白与RxLR31197互作的抗病分子机理，推动认识葡萄-病原卵菌互作的分子机制。

Grape Downy Mildew, caused by the pathogen Plasmopara viticola, is one of the most devastating grapevine oomycete diseases worldwide. RxLR effectors play important roles in the interactions between oomycete and plants. Our previous transcriptomic analysis found an RxLR effector, which named RxLR31197. At present study, a PVX-based high-through plant expression system which can block the programmed cell death induced by mouse pro-apoptosis protein Bax and can trigger cell death in Nicotiana benthamiana was used and the key recognition and subcellular localization were analysed to clarify the toxicity and functional site. In the following study, a cDNA library, constructed using diseased leaf tissues of Chinese Wild Vitis. piasezkii ‘Liuba-8’ infected with P. viticola, were screened for proteins interacting with RxLR31197 using a yeast two-hybrid approach. The goals are to identify resistant genes of host plant interacting with RxLR31197 and to investigate on the interaction between oomycete pathogens and their host plant and the disease resistance mechanism at the molecular level. This study facilitates further investigation on translocation of effector and its virulence mechanisms in host plant cells and will facilitate the development of novel disease control strategies.