葡萄霜霉病是由葡萄霜霉菌引起的、危害葡萄生产的一大卵菌病害。RxLR效应蛋白在卵菌与植物互作中发挥着重要作用。申请人前期从葡萄霜霉菌基因组中筛选到了一个RxLR效应蛋白（PvRxLR110）。初步的功能分析表明PvRxLR110在烟草和葡萄中均能促进病原菌的定殖，是一个重要的毒性因子。在此基础上，本项目首先以PvRxLR110为诱饵，筛选验证其在感病葡萄‘无核白’中的靶标蛋白，并研究PvRxLR110与靶标蛋白的互作关系。然后对前期已经获得的PvRxLR110过表达转基因葡萄植株进行RNA高通量测序，分析‘无核白’响应PvRxLR110的关键代谢通路及与靶标蛋白的关系。最后构建靶标蛋白过表达和CRISPR敲除载体，转化‘无核白’，在转基因葡萄中明确靶标蛋白的功能。揭示PvRxLR110与靶标蛋白互作的分子机理，推动认识葡萄-霜霉菌互作的分子机制，为葡萄霜霉病防治和抗病分子育种提供理论依据。

Grape downy mildew, caused by Plasmopara viticola, is one of the most devastating grapevine oomycete diseases worldwide. RxLR effectors play vital roles in the interaction between oomycetes and plants. PvRxLR110, a RxLR effector identified from Plasmopara viticola genome, was found to be crucial in promoting the colonization of pathogens in tobacco and grapevine. On this basis, the potential targets of PvRxLR110 in the susceptible Vitis vinifera cv. ‘Thomson seedless’ will be first screened and the relationship between their interactions will be explored. Then, the key metabolic pathways in response to PvRxLR110 and their relationship with the targets will be investigated in the ‘Thomson seedless’ line overexpressing PvRxLR110 through RNA high-throughput sequencing. Finally, the biological function of the targets will be elucidated by their gene overexpression and CRISPR knockout in ‘Thomson seedless’ plants. This study will reveal the molecular mechanism of the interactions between PvRxLR110 and its targets, which will promote the understanding of the relationships between grapevine and Plasmopara viticola and will provide a theoretical basis for grape downy mildew prevention and molecular breeding for disease resistance.