急性肝衰竭发病急、死亡率高，救治难度大。一直以来，广西都是病毒性肝炎的高发区，肝衰竭的发病率明显高于全国平均水平。肝细胞再生是肝衰竭研究领域里具有重要价值和应用前景的研究方向。有研究表明JAK/STAT 信号通路可能是影响肝细胞再生的重要机制，大黄、赤芍是治疗肝衰竭的要药，其在肝衰竭治疗中起着非常重要的作用，分子生物学技术的发展为进一步明确肝细胞再生功能基因及特异性调控提供了有力的研究保障。本项目拟利用基因芯片、western blot、RT-PCR 等技术研究 JAK/STAT 信号通路在肝细胞再生中的传导途径，阐明肝衰竭肝细胞再生过程中 JAK/STAT 信号通路变化特点，及大黄赤芍是否通过调节该通路来发挥促进肝细胞再生的作用机制，为治疗肝衰竭提供重要的理论和技术支持。

The symptoms of AHF（acute hepatic failure ）is onset urgent,with treatment difficult and high mortality.All this time,GuangXi is considered to be a high incidence area of viral hepatitis,since the morbidity of acute liver failure is above the national average .Liver cell regeneration creates very important value and application prospect in ALF research field.Many studies shows that JAK/STAT signal pathway could be a critical mechanism has effects at liver cell regeneration,Rhubarb and Red peony root play very important role in acute liver failure treatment,The development of molecular biological technique provides supporting in the further definite of liver cell regeneration functional gene and specific regulation.This project intends to indicate the relation of characteristics change of JAK/STAT signal pathway,and Rhubarb,Rred peony root would or would not progresses liver cell regeneration process,associated with DNA microarray,western blot,RT-PCR,and so on,as well as making theoretical and technical support at ALF treatment.