长链非编码RNA（LncRNA）与肿瘤发病关系密切，但乳腺癌相关的LncRNA研究甚少。前期我们在乳腺癌标本中经过芯片杂交筛选出了有显著差异的LncRNA 500余条并预测靶mRNA，选择LncRNA-NR002734(本课题组首次发现,自命名为BCL1)及其预测靶基因PTTG1进行了研究，现已初步验证。下一步①大样本乳腺癌标本检测BCL1表达、分析其与临床恶性度的相关性。②乳腺癌细胞实验：干扰BCL1表达观察对细胞生物学行为的影响并研究BCL1与PTTG1的调控机制。以期初步阐明BCL1在乳腺癌发病中的作用。

Long non-coding RNA（LncRNA） is a major finding in the contemporary life science, it is reported that long non-coding RNA（LncRNA ）is closely related with tumorigenesis, however, breast cancer associated LncRNA has not been researched extensively and deeply. We screened more than 500 differentially expressed LncRNAs and predicted target mRNAs in breast cancer specimens through latest LncRNA microarray and bioinformatics analysis in the preliminary experiments. BCL1（first discovered, self-named）and its predicted target gene PTTG1 were selected for the further research and have been validated primarily. Next, we intend to: 1)detecting the expression of BCL1 and PTTG1 in breast cancer with a larger sample size and analyzing its correlation with clinical malignant degree; 2)breast cancer cell line experiments: investigating the effect of BCL1 on cellular biological behavior with RNA interference technique;exploring the relationship between BCL1 and PTTG1 and their mechanisms.We hope to clarify the role and mechanism of BCL1 in the development of breast cancer preliminarily。