研究表明，氧化应激是非酒精性脂肪性肝病（NAFLD）的重要发病机制之一；Nrf2/ARE通路是迄今发现最为重要的体内抗氧化系统；MAPKs对Nrf2/ARE通路具有重要的调控作用。我们前期研究发现，疏肝健脾方药能显著抑制NAFLD大鼠肝细胞、Kupffer细胞MAPKs等作用而发挥良好的防治效果，但具体机制不明。研究目的：体内、体外动态观察p38MAPK介导NAFLD大鼠肝细胞、Kupffer细胞Nrf2/ARE通路对NQO1、GST、HO-1、CAT等氧化应激相关基因的调控作用及疏肝健脾方药干预的分子机制；预期：①明确p38MAPK介导Nrf2/ARE通路在NAFLD病理演变过程中对氧化应激相关基因的调控机制；②揭示疏肝健脾方药对p38MAPK介导NAFLD大鼠Nrf2/ARE通路及氧化应激相关基因的干预作用；③发现中药作用的新靶点；④丰富NAFLD的中医药防治理论和现代发病机制。

Researches indicate that oxidative stress is one of the central parts to the pathogenesis of nonalcoholic fatty liver disease (NAFLD)；Nrf2/ARE pathway is the most important antioxidant system in vivo；MAPKs play an essential role in regulating Nrf2/ARE pathway. In our previous studies, soothing liver and invigorating spleen recipes have a significantly preventive and curative effect on NAFLD through inhibition of MAPKs activation in hepatocytes and Kupffer cells of rats. However the specific mechanism remains unclear... Objective：To dynamically observe the role regulating effect of Nrf2/ARE pathway mediated by p38MAPK in the regulation of oxidative stress-related genes, such as NQO1,GST,HO-1,CAT, etc. in hepatocytes and Kupffer cells of NAFLD rats in vivo and in vitro, and to investigate the effect of the molecular mechanism of soothing liver and invigorating spleen recipes...Anticipation:.①To observe the mechanism of Nrf2/ARE pathway mediated by p38 MAPK regulating oxidative stress-related genes in pathologic processes of NAFLD..②To observe the effect of recipes of soothing liver and invigorating spleen on Nrf2/ARE pathway mediated by p38 MAPK and oxidative stress-related genes of NAFLD rats..③To find the new drug targets of Chinese medicine..④To enriches the theory of preventing and treating NAFLD with traditional Chinese medicine and modern concepts of the pathogenesis of NAFLD.