研究表明：①肠源性内毒素血症（IETM）在NAFLD的发生发展中具有重要作用。②炎症是单纯性脂肪肝向NASH甚至肝硬化、肝癌发生发展的关键因素。③有交汇协同作用的TLR4和NLRP3通路与NAFLD发生发展密切相关。我们研究发现，参苓白术散对NAFLD的防治效果肯定，其对有关炎症因子的调控作用可能是该方取效的重要机制之一，但详细作用机制未明。本项目采用高脂饮食诱导大鼠NAFLD模型，运用16S rDNA测序、Western Blot、PCR等技术，观察参苓白术散对NAFLD大鼠肠道微环境、IETM的作用及肝细胞、Kupffer细胞TLR4/NLRP3通路和炎症因子的影响。预期：揭示参苓白术散抗NAFLD作用的分子机制。

It is reported that: ① Intestinal endotoxemia (IETM) plays an important role in the development of NAFLD. ② Inflammation is a key factor in the development of hepatocellular steatosis to NASH and cirrhosis. ③ There intersection between the TLR4 and NLRP3 pathway is closely related to the development of NAFLD. We found that Shenling Baizhu Powder had a definite effect in the treatment of NAFLD, and its mechanism may be through inhibition of hepatic inflammatory factors, but the detailed mechanism is unknown. We plan to use high-fat diet induced rat model of NAFLD in experimental models, and use various methods including 16S rDNA sequencing, Western Blot, and Quantitative Real-time PCR. We will take further experiments to observe intestinal microenvironment, endotoxemia, TLR4/NLRP3 pathways in hepatocytes and Kupffer cell and its role of related inflammatory cytokines on NAFLD treated with Shenling Baizhu Powder in vivo. Thereby the new molecular mechanisms of Shenling Baizhu Powder in treatment of NAFLD may be well illuminated.