胃黏膜疾病严重影响人们的健康及生活质量，对其发病机制进行研究有助于疾病的有效防治。CMTM家族作为一种膜泡运输相关蛋白，与机体免疫功能调节和疾病发生可能相关。我们前期研究发现，幽门螺杆菌（Hp）感染后CMTM4表达下调；CMTM4在胃癌组织中表达下调，CMTM4的表达水平与胃癌预后存在显著正相关。本项目将进一步通过细胞实验干预CMTM4表达，检测Hp感染胃黏膜细胞的增殖、凋亡、迁移以及炎性细胞因子的表达，用蛋白质组学方法探索CMTM4下游分子机制；构建Cmtm4基因敲除小鼠自发胃黏膜疾病模型，评价CMTM4表达对胃黏膜疾病发生发展的影响，用蛋白质组学方法进一步探索CMTM4的作用机制；利用临床组织分析CMTM4及其下游分子表达与胃黏膜疾病类型、Hp感染等临床资料的相关性。以揭示CMTM4在胃黏膜致病过程中的作用及机制，为有效防治胃黏膜疾病、寻找阻断胃癌发生的新靶点提供理论依据。

Gastric mucosal diseases seriously affect people's health and quality of life. Studying on the pathogenesis is helpful for effective prevention and treatment of gastric mucosal diseases. CMTM family, as a membrane vesicular transport-related protein, may be involved in regulating immune function and diseases occurrence. Our previous studies showed that CMTM4 expression was down-regulated after helicobacter pylori (Hp) infection. CMTM4 was down-regulated in gastric cancer tissues, and the expression level of CMTM4 was positively correlated with the prognosis of gastric cancer. In this project, we will further research on CMTM4 by intervening CMTM4 expression in cell experiment, followed by detecting gastric mucosa cell proliferation, apoptosis, migration infected with Hp, measuring inflammatory cytokines expression, and exploring the downstream molecular mechanisms of CMTM4 by proteomic analysis. The cmtm4 gene knockout mice model of spontaneous gastric mucosal disease will be established, and the effects of CMTM4 expression on the development of gastric mucosal disease will be evaluated, and proteomic analysis will be performed on animal tissues. In clinical tissues, the relationship between CMTM4 and its downstream molecules expression and clinical data such as gastric mucosal disease type and Hp infection will be analyzed. This project will reveal the roles and mechanisms of CMTM4 in the pathogenesis of gastric mucosa, and provide theoretical basis on identifying new targets for prevention, early diagnosis and treatment of gastric mucosa diseases, especially gastric cancer.