在确证板蓝根水提取物中存在具有不同抗病毒谱活性成分，并发现活性成分为以往未报道新型微量成分的基础上，通过活性与色谱-波谱联用相结合跟踪，运用组合闪式色谱与全自动制备HPLC以及超低温探头NMR等高效率分离和结构鉴定方法，从锁定的活性组分中，寻找并分离获取新型活性分成，研究其结构和活性特点；并以板蓝根中新型抗病毒成分生源合成分析为指导，以较高含量关联成分为前体，设计合成不同骨架系列衍生物，结合活性测定，研究构效关系，获得先导化合物。同时，运用细胞和酶水平多种抗病毒药理模型，评价不同活性成分的抗病毒谱特征，探讨它们的抗病毒作用机制。该项目在板蓝根微量新成分结构、生源指导的活性化合物转化合成、构效关系、抗病毒谱特点和作用机制等方面，有重要学术意义；在基于板蓝根抗病毒活性成分的新型药物研究方面，有实际应用价值。

Based on verifying the presence of active constituents different antiviral spectra and discovery that the active constituents are unknown minor coponents in the water extract of the root of Isatis indigotica , under guidance of antiviral bioassay combinded with chromatogarophic and spectroscopic detection, this project will carried out the isolation, structural characterization, and bioactivity evaluation of the active constituents with novel skeletons from the focused active fractions by using Combiflash chromatography and auto-HPLC separation and super low temperature probe NMR structural characterization techniques. With ananlysis of biosynthetic pathways of the active constituents, different types of analogues will been designed, semisynthesized,and assayed to investigate structure-activity relationships and obtain lead compounds by using co-occuring higher aboundant biosynthetic procursors. In addition, the active constituents will be evaluated on various cell- and/or enzyme-based models to evaluate characters of antiviral spectra and to explore mechanisms of the antiviral activities. This project has a creative scientific importance in structures of the minor antiviral components with novel skeletons from Isatis indigotica, as well as in transformation synthesis conducted by analysis of biosynthesis pathway, structure-activity relationships,characters of antivaral spectra, and the mechanisms of the active compounds. The project also has a potential application value in antiviral drug discovery and development based on the active components from Isatis indigotica.