如何提高胚胎着床率是困扰生殖专家们的世界性难题，为限制辅助生殖技术成功的瓶颈问题。我们的青年基金研究对HOX通路在胚胎着床中的作用进行初步探索，发现了13种可能参与胚胎着床的HOX通路下游基因。本项目拟以临床上最具代表性的几种IVF促排方案为主线，通过探索新发现的HOX通路分子与胚胎着床的关系，寻找可能影响IVF胚胎着床的关键性基因，并采用RNA干扰技术和宫腔内脂质体转染模型以进一步验证靶基因功能，旨在破译HOX通路调控胚胎着床的分子机制、探索可运用于临床的、最具代表性的子宫内膜容受性特殊标志物；另一方面，本项目通过比较不同的IVF促排方案对胚胎着床及子宫内膜容受性特殊标记物表达的影响，从而从内膜水平进一步明确不同促排方案之间的差异，为临床上IVF促排方案的选择提供有力的证据支持。这项工作除了IVF超促排卵对内膜容受性的影响提供新的认识外,特殊标志物的识别还能为新的靶基因治疗技术提供可能.

How to enhance embryonic implantation rates of IVF have always been a difficult problem for the reproductive experts，which represents the bottle - neck to restrict the enhancement of ART pregnancy rates. We have explored the mechanism of HOXA10 and cofactors regulating embryonic implantation in the early studies. Our project aim to research the relationship between the new proved genes target genes and embryonic implantation in IVF ovarian stimulation cycles, further clarify the HOX downstream paths by EMSA and Luciferase Reporter Gene Assay, and confirm the function of these target genes in embryonic implantation by gene transfection and siRNA techniques, so as to demonstrate the differences between each ovarian stimulation protocols in the level of endometrium, explore the special molecular marker of endometrial receptivity which could be used in clinic. The identification of these marker genes related to embryonic implantation could lay the foundations for gene therapy, which contribute to increase IVF achievement ratio and promote the development of ART.