炎症过程中白细胞在炎症部位的募集是由一系列黏附分子介导的，其中表达于白细胞表面的P-选择素配体(PSGL-1)主要介导了白细胞的起始黏附，而由此引发的稳定黏附则主要依赖于整合素(Integrin)的作用。脂筏(Lipid raft)是细胞膜上富含胆固醇和鞘磷脂的动态微区结构，参与了细胞黏附、迁移和活化等很多的生理事件。目前对于脂筏结构在白细胞由起始黏附至稳定黏附动态转变过程中的作用还不清楚。根据已有的文献资料和我们前期的一些实验结果，我们推测脂筏结构作为白细胞膜上一个蛋白质停泊的平台参与了PSGL-1介导的β2-整合素活化和白细胞稳定黏附。本研究将①确定脂筏结构在PSGL-1介导的β2-整合素活化和白细胞稳定黏附中的作用；②筛选和鉴定脂筏结构中能够调节PSGL-1功能的蛋白分子；③解析这些蛋白分子参与调节PSGL-1介导的β2-整合素活化和白细胞稳定黏附的分子机制。

The recruitment of leukocyte at inflammation sites is mediated by a series of adhesion molecules. Among them, P-selectin glycoprotein legand-1 (PSGL-1) , expressed on the surface of leukocyte, play a critical role for the cell initial adhesion, and the followed stable adhesion is dependent of Integrins. Lipid raft is a dynamic minor structure on cell surface, which is full of cholesterol. Lipid raft has been reported taking part in cell adhesion, cell migration and cell activation. It is still not clear until now that whether the lipid raft structure functions during the dynamic change of leukocyte from initial adhesion to stable adhesion. Based on the previous published data and some of our experiment results, we inferred that the lipid raft, as a platform of anchoring proteins on the surface of leukocyte, participated in the PSGL-1 mediated β2-integrin activation and leukocyte adhesion. In this study, we will ① determ the role of lipid raft in PSGL-1 mediatedβ2-integrin activation and leukocyte stable adhesion, ② screen and identify the proteins which can regulating the function of PSGL-1 in the lipid raft structure, ③ closely examin the molecular mechanism of the proteins in regulating the PSGL-1 mediatedβ2-integrin activation and leukocyte stable adhesion.