骨骼因其精细结构和优良机械性能而受到广泛关注，其主要成分是磷灰石晶体、胶原基质和非胶原蛋白。目前骨骼中磷灰石晶体分布在胶原基质内部或外部仍存在争议。因此，在体外实现单根胶原纤维内部或外部的可控矿化对进一步了解骨骼的结构特征将非常有意义。本项目通过学习骨骼形成过程中非胶原蛋白作用，设计和构建多功能重组蛋白以模拟非胶原蛋白的功能，体外指导磷灰石在胶原纤维内有序矿化，并探究矿化机制及动力学过程。本项目将揭示无定形磷酸钙进入胶原的驱动力，系统研究重组蛋白与材料界面效应指导胶原纤维内磷灰石有序生长的规律，阐明磷灰石在胶原内晶型转变、生长速率等矿化动力学过程。最终为实现胶原纤维内矿化的可控制备奠定理论基础，在一定程度上揭示骨矿化机制，并推动骨组织再生与修复技术的发展。

Bone has attracted wide attention due to its exquisite structure and superior mechanical properties. It is composed of apatite crystals, collagen matrix and noncollagen proteins. At present, it is still controversial that apatite crystals distribute inside or outside collagen matrix. Therefore, controlling the synthesis of isolated collagen fibers with internal or external mineralization in vitro will be very meaningful to infer the structural characteristics of bone. In this project, we design and construct recombinant proteins to mimic the function of non-collagen proteins through studying the process of bone formation, and regulate the organized mineralization of apatite inside collagen fibrils in vitro, then explore the mechanism and kinetics of mineralization. We will reveal the driving force of amorphous calcium phosphate infiltrating into collagen; and systematically study the interfacial effect between recombinant proteins and minerals; and clarify the mineralization kinetics of apatite in collagen, such as crystalline transformation and growth rate. Finally, the results will facilitate the controllable synthesis of mineralized collagen fibrils and reveal the mechanism of bone mineralization to a certain extent, and promote the development of bone tissue regeneration and bioremediation.