我们研究证实活血安心方改善大鼠心肌梗死后心室重构，并调控STIM1/ORAI1钙信号通路转导异常的新机制；本研究拟首先确认乳鼠心肌细胞STIM1/ORAI1相互作用的lncRNA MEG3，观察在miR-223过表达或干扰下活血安心方干预苯肾上腺素,血管紧张素2刺激的心肌细胞表面积、利钠肽水平及Ca2+浓度、对心肌细胞ICa-L、细胞内钙瞬变、肌浆网钙泄漏和早期后除极心律失常的发生及MEG3, STIM1/ORAI1表达异常的差别；进而研究心梗后心衰大鼠活血安心方干预后，lncRNA MEG3调控miR-223，及MEG3,STIM1/Orai1水平及定位表达的差别，及激光共聚焦-膜片钳技术检测心肌细胞钙电流的作用，探索活血安心方的lncRNA MEG3/MIR-223/STIM1轴钙信号转导通路影响心室重构，改善心功能，减少恶性心律失常发生的新靶向调控机制，为临床应用研究提供依据。

Our study confirmed that Huoxue Anxin Recipe(HAR) can improve ventricular remodeling and regulate abnormal STIM1/ORAI1 calcium signaling pathway after myocardial infarction. In this study, we’ll first confirmed the interaction between STIM1/ORAI1 in neonatal rat cardiomyocytes by LNC RNA MEG3, and observed the intervention of HAR on phenylephrine, angiotensin-2-stimulated cardiomyocyte surface area and natriuretic peptide level under the over-expression or interference of micRNA-223. The differences of Ca2+ concentration, intracellular calcium transients, intracellular calcium leakage of sarcoplasmic reticulum, early depolarization arrhythmia and abnormal expression of MEG3, STIM1/ORAI1 in myocardial cells were studied. Then, after intervention with Huoxue Anxin Formula in rats with post-infarction heart failure, the differences of expression and localization of microRNA-223, MEG3, STIM1/Orai1 regulated by LNC RNA-MEG3, and laser confocal-patch clamp technique were studied to detect myocardium. To explore the effect of calcium current on Lnc RNA MEG3/MIR-223/STIM1 axis calcium signal transduction pathway of HAR on ventricular remodeling, improve cardiac function, reduce malignant arrhythmia and provide a basis for clinical application.