橡胶树乳管组织合成和贮存的天然橡胶是全球所需天然橡胶的主要来源。通过切割树干树皮，使胶乳（提炼天然橡胶的原料）从乳管伤口处排出。在排胶持续一定时间后，乳管伤口被堵塞，导致排胶停止。由于排胶持续时间是直接决定天然橡胶产量的关键因素，所以，乳管伤口堵塞机制是天然橡胶生产面临的一个重大理论问题。我们发现胶乳过敏原蛋白Hev b7是有效的橡胶粒子去凝集因子，乙烯利刺激显著延长排胶时间主要是通过增加Hev b7含量而延缓了橡胶粒子的凝集。目前对Hev b7拮抗橡胶粒子凝集因子的生化机制仍不清楚。本项目拟采取蛋白质分离纯化、突变体蛋白制备以及酵母双杂交、SPR和Pulldown等蛋白质互作分析相关技术，鉴定橡胶粒子上与橡胶粒子凝集促进因子和抑制因子结合的靶标蛋白及其互作区段和主要位点以及促进因子或抑制因子之间的互作分析，揭示橡胶粒子凝集和去凝集的调控机制，对于改良橡胶树的排胶性状也具有重要的指导意义。

The natural rubber that is biosynthesized and stored in laticifers of rubber trees meets the most demands for natural rubber in the world. The latex (the resource of refining natural rubber) flows out of the severed laticifers after the trunk bark being cut. The latex flow lasts for several times and thereafter terminates as the result of the plug formation at the end of the severed laticifers. Considering that duration of latex flow is a key factor that directly determines the rubber yield per tapping, the mechanism of laticifer plugging is a major theoretical subject that is faced by the natural rubber production. We have discovered that latex allergen Hev b7 is effective in inhibiting rubber particle aggregation and the ethrel-caused prolongation of the duration of latex flow is associated with the increase in the level of the Hev b7 that hinders rubber particle aggregation. It is so far unknown about the biochemical mechanism for the antagonistic effect of allergen protein Hev b7 on rubber particle aggregating factors. This project is to identify the target protein on the rubber particles that could interact with the promoting and inhibiting factors of rubber particle aggregation, the interact region and major amino acid within the region as well as the interaction between the promoting factors or inhibiting factors by using the biochemical techniques such as protein isolation and purification, preparation of mutant proteins, yeast-2-hybridization, SPR and Pulldown, so as to reveal the regulating mechanism for rubber particle aggregation and deaggregation. The results are also significant for improving the latex flow traits.