金属修复体传统的制作方法为失蜡铸造法，如今选择性激光熔融和数字化切削等数字化成型方法也已广泛应用于临床。迄今不同成型方法的金属修复体金属离子在局部和全身蓄积和代谢情况仍知之甚少。前期研究发现铸造牙科合金可影响口腔粘膜、血中金属离子蓄积，并引起以凋亡为主的细胞死亡，激活自噬相关因子，但数字化成型方法是否影响金属离子析出情况与生物相容性还有待研究。为此，本项目拟：（1）研究不同数字化成型的牙科合金表面性状、对细胞的生物学效应及其机制（2）构建比格犬上颌金属支架修复模型，研究戴用及摘除后不同数字化成型的金属修复体后对犬粘膜、血清及肝肾中金属离子种类和浓度及生物学变化的影响。从而对比分析不同数字化成型的金属修复体表面性状及其戴用时长对金属离子在口腔局部与全身中的变化及转归，及其引起局部与全身病理变化的机制。此外，本项目的研究结果也将为临床诊疗过程中不同加工方式金属修复体的应用提供参考依据。

With the promotion and application of digital technology in the dentistry, increasingly digital molding methods such as selective laser melting(SLM) and CAD/CAM appear in the molding methods of dental alloys in addition to the traditional lost wax casting process. So far the accumulation and metabolism of metal ions released from metallic dental prosthesis that processed by different molding methods still remains unknown.Our previous studies have proved that metallic ions released from dental casting alloys can accumulate in oral mucosa and peripheral blood, which might cause apoptosis-based cell death via the mitochondrial pathway and trigger the autophagic regulator. However, whether different molding methods affect the biocompatibility of dental alloys needs to be further studied . The present study aims to (1) observe the surface characteristics of the dental digitization molding alloys as well as the type and concentration of metal ions released from the cobalt-chromium alloys and commercial pure titanium processed by lost wax casting process, selective laser melting(SLM) and CAD/CAM ,and evaluate biological effects and mechanism by different concentration solution of single metal ions on dog gingival fibroblasts cells (2) establish a beagle canine maxillary mental plate model, and investigate the concentration and biological change of metal ions in the palatal mucosa, peripheral blood, liver and kidney with and without removal of the plate. The findings would extremely valuable in comparative analyzing the effect and outcome of the metal ions in the oral local region and the whole body by different dental digitization molding alloys as well as wearing duration, in order to provide an experimental evidence for alloys choosing of different molding methods in dental clinic.