长距离运动是植物病毒系统侵染寄主的关键环节。番茄斑萎病毒属病毒是多种经济作物的重要病原，其长距离运动机制仍不清楚。番茄环纹斑点病毒（TZSV）为云南及周边地区的优势种，前期研究发现感病植物的导管和筛管中不存在TZSV病毒粒体，推测TZSV在输导组织中长距离运动形式为vRNP复合体或囊泡相关复制酶复合体(VRC)。本项目拟利用显微技术、免疫标记技术等明确TZSV长距离运动形式；利用蔗糖密度梯度离心、BN-PAGE分离纯化导管或筛管汁液中的vRNP复合体或囊泡相关VRC，质谱鉴定其组分中的病毒蛋白及寄主蛋白；利用免疫共沉淀、BiFC明确病毒蛋白与寄主蛋白互作关系；通过上调、下调表达寄主蛋白，鉴定对TZSV长距离运动起关键作用的寄主蛋白，分析寄主蛋白对TZSV长距离运动的影响。提出TZSV在输导组织中的运动模型，分析TZSV长距离运动机制，为病害防控提供理论依据。

Long-distance movement is an essential step for systemic infection of plantviruses. Still it is not clear that how tospoviruses carry out the long-distance movement. Tomato zonate spot virus (TZSV), is one of the most important tospovirus, pathogenic to various economic crops in Yunnan and around regions. Our previous study showed that no virion had been found in the vessels and sieve tubes of conducting tissue in the TZSV infection. We suggested that TZSV viral form of long-distance movement might be vRNP complex or vesicle-related viral replicase complex (VRC). In this study, we would use microtechnology and immunolabelling technique to analyze viral form used for long-distance movement; Use sucrose density gradient centrifugation and BN-PAGE technology to concentrate and purify vRNP complex or vesicle-related VRC, and LC-MS/MS method to identify the components of these complexes; Use Co-IP, BiFC to detect interactions between virus proteins and host proteins; Use overexpression and down-regulated expression to analyze the effects of host proteins on systemic infection of TZSV, finally identify the host proteins which play a crucial role on TZSV long-distance movement. The model of TZSV long-distance movement will be proposed，and the mechanism of long-distance movement of tospoviruses will be analyzed. These results will provide the theoretical basis for plant disease control.