近年来，随着生殖系统发育异常形势的日益严峻和人们对之认识的逐步加深，睾丸作为核心器官其发育备受关注。睾丸的发育伴随着两个下降过程，其异常发育与下降密切关联。既往研究表明胰岛素样因子3（INSL3）在睾丸经腹下降期起主导作用，且与其中重要的引导性结构：睾丸引带的发育关系密切。我们前期研究也证实INSL3特异性受体：松弛素家族肽受体2（RXFP2）存在于小鼠睾丸引带细胞（GTCs）上，INSL3可影响GTCs的形态结构及增殖和收缩活性，且与RXFP2密切相关。目前对RXFP2的了解仍有限，对INSL3影响睾丸引带发育的作用机理也很不清楚。结合肽类激素可直接作用于其受体，激活胞内第二信号系统而发挥生物学效应的机理研究，本项目拟通过培养GTCs，基于PKA和PKC信号途径探讨RXFP2介导INSL3影响GTCs发育的可能机制，以深化INSL3及RXFP2在睾丸下降甚至整个雄性生殖系统发育中的作用。

In recent years, with the increasingly severe development of the reproductive system and the deepening of people's understanding of it, the development of testis as the core organ has attracted much attention.The development of testis is accompanied by two descending processes, and its abnormal development is closely related to the decline.Previous studies have shown that insulin-like factor 3 (INSL3) plays a leading role in the first transabdominal phase of testicular descent, and is closely related to the important guiding structure: the development of gubernaculum. Our previous studies have confirmed INSL3 specific receptors: relaxin family peptide receptor 2 (RXFP2) exists in mice gubernaculum testis cells (GTCs), INSL3 can affect the morphology and proliferation and contraction of the GTCs, and is closely related to RXFP2. At present, the understanding of RXFP2 is still limited, and the mechanism of INSL3 affecting the development of testis is also unclear. Peptide hormone binded its receptors could produce biological effect through signal transduction pathway. So, we explore the value of RXFP2 in the mechanism of INSL3 influence mice gubernaculum cell development via PKA and PKC signaling pathway in vitro.