RXFP2 as novel potential biomarker for abnormal differentiation induced by diethylstilbestrol in the gubernaculum of fetal mice.
RXFP2 as novel potential biomarker for abnormal differentiation induced by diethylstilbestrol in the gubernaculum of fetal mice.
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RXFP2 作为己烯雌酚在胎鼠脐带中诱导异常分化的新型潜在生物标志物。
DOI：
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发表时间：
2020-07
影响因子：
2.2
通讯作者：
Shuhua Ma
中科院分区：
文献类型：
--
作者：
Shouxing Duan;Xuan Zhang;Xuewu Jiang;Yao Xie;Lian Zheng;Bingna Zhang;Wenfeng Xiao;Xinquan Xie;Xiaojun Xie;Jianhong Li;Shuhua Ma
环境雌激素（EEs）与男性泌尿生殖系统的异常有关。然而，这些分子的作用机制仍不清楚。采用体内外实验研究了乙烯雌酚（DES）对胎鼠引带松弛素家族肽受体2（RXFP 2）表达的影响及其机制。体内实验结果表明，DES治疗逐渐增加死胎率，显着减少引带锥体积，并破坏组织结构，导致睾丸下降不完全。体外实验表明，DES给药导致引带细胞形态异常和结构紊乱，以剂量依赖的方式失去其原有的形态。此外，DES诱导的F-肌动蛋白重排和应力纤维的形成在培养的细胞。蛋白定量分析显示，各实验组RXFP 2水平均显著低于正常组。结论：DES影响了睾丸引带的形态学，改变了引带的结构，并影响了RXFP 2蛋白的表达。这些数据表明DES对胎鼠引带具有毒性，并且RXFP 2与DES诱导的引带形态异常相关。总之，这些数据表明，RXFP 2可能是一种新的潜在的生物标志物异常分化的引带。
Environmental estrogens (EEs) have been correlated with abnormalities in the male urogenital system. However, the mechanism underlying the effect of these molecules remains unclear. In vitro and in vivo experiments were performed to examine the expression level and mechanism of relaxin family peptide receptor 2 (RXFP2) in the gubernaculum of fetal mice following diethylstilbestrol (DES) treatment. The in vivo results demonstrate that DES treatment increased the stillbirth rate gradually, decreased the gubernacular cone volume significantly, and disrupted the tissue structure, leading to incomplete testicular descent. In vitro experiments reveal that DES administration resulted in abnormal cellular morphology and structural disorder of gubernacular cells, which lost their original morphology in a dose-dependent manner. Moreover, DES-induced F-actin rearrangement and stress fiber formation in cultured cells. Protein quantitative analysis showed that the RXFP2 level in each experimental group was significantly lower than that of the normal group. In conclusion, DES affects the morphology and alters the gubernaculum structure, as well as the expression of RXFP2 protein. These data demonstrate that DES is toxic to gubernaculum in fetal mice, and that RXFP2 is associated with the abnormal gubernaculum morphology induced by DES. Taken together, these data suggest that RXFP2 may be a novel potential biomarker for abnormal differentiation of the gubernaculum.
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影响因子：
13.8
作者：
Wong EW;Cheng CY
通讯作者：
Cheng CY
影响因子：
2.9
作者：
Ivell, Richard;Heng, Kee;Anand-Ivell, Ravinder
通讯作者：
Anand-Ivell, Ravinder
DOI：
10.1002/jez.b.22637
发表时间：
2015-11
期刊：
Journal of experimental zoology. Part B, Molecular and developmental evolution
影响因子：
--
作者：
Aldo Donizetti;M. Fiengo;R. del Gaudio;G. Iazzetti;Paolo Pariante;S. Minucci;F. Aniello
通讯作者：
Aldo Donizetti;M. Fiengo;R. del Gaudio;G. Iazzetti;Paolo Pariante;S. Minucci;F. Aniello
影响因子：
2.9
作者：
Xue-Wu Jiang;Jian-hong Li;Tian-hua Huang;Wang-Dong Deng
通讯作者：
Xue-Wu Jiang;Jian-hong Li;Tian-hua Huang;Wang-Dong Deng
影响因子：
3.7
作者：
Favorito, Luciano A.;Costa, Suelen F.;Sampaio, Francisco J. B.
通讯作者：
Sampaio, Francisco J. B.