艾灸有效保护和修复血管ECs，治疗心脑血管疾病，离不开Trpv1通路的皮肤启动和血管响应，其中，艾灸保护和修复血管ECs的分子生物学机制，尚不清晰。另一方面，血管ECs是维持血管正常功能的基础、炎症反应和氧化应激等是ECs损伤的主要病理机制；激活Trpv1、调控PI3K/Ak相关信号通路，可以控制炎症反应、降低氧化应激，有效促进ECs保护和修复，预防和治疗心脑血管疾病。本项目拟以艾灸激活Trpv1促进血管ECs保护和修复为切入点，利用形态学、生物化学、免疫细胞化学、免疫组织化学、蛋白免疫印迹法及RT-PCR等细胞生物学技术和分子生物学技术等，通过体内、体外相互印证，研究艾灸激活Trpv1、通过调控PI3K/AKT信号通路以促进血管ECs自我保护和修复的内源性调节机制，阐明艾灸温通血脉的内在主要机制和关键科学内涵，为艾灸诊治心脑血管性疾病提供现代依据。

Moxibustion can effectively treat cardiovascular and cerebrovascular diseases by protecting and repairing vascular ECs, which is inseparable from the skin activation and vascular response of Trpv1 pathway. The molecular biological mechanism of them is still unclear. On the other hand, vascular ECs is the basis of maintaining normal vascular function, and inflammatory reaction and oxidative stress are the main pathological mechanisms of ECs injury. Activating of Trpv1, regulating of PI3K/Akt pathway can control inflammatory reactions, reduce oxidative stress, effectively to protect and repair ECs, and to prevent and treat the cerebrovascular diseases. .From clinical and laboratory ways, focusing on activating Trpv1 to protect and repair ECs by moxibustion, this project will research the molecular biological mechanism of moxibustion to activate Trpv1 form skin to vessel, and to regulate the PI3K/AKT/eNOS signaling pathway by the techniques of cell biology and molecular biology, such as morphology, biochemistry, immunocytochemistry, immunohistochemistry, protein western blot and rt-pcr, were used to verify each other in vivo and in vitro. The result of this research will reveal the endogenous regulatory mechanisms to promote vascular ECs self-protection and repair by moxibustion, clarify the internal mechanism and key scientific connotation of Wentongxuemai, and also provide a modern basis for treating cardio-cerebral vascular disease by moxibustion.