本项目基于我们长期针灸治疗脑缺血的临床和实验研究工作基础，结合目前外泌体miRNAs与脑缺血后血管新生之间相互关系的研究进展，本项目拟从在体和离体实验探讨电针调控外泌体miR-210促进脑缺血后血管新生的机制。通过建立大鼠局灶性脑缺血再灌注模型和人脐静脉内皮细胞缺氧模型，借助透射电镜、激光共聚焦、纳米颗粒跟踪技术、免疫组织化学、western blot、Real Time PCR、siRNA、细胞转染等技术分析电针、外泌体miR-210、HIF-1、VEGF及Notch的表达之间的关系，从外泌体miR-210调节HIF-1/VEGF/Notch信号通路角度阐明电针促进脑缺血后血管新生的调控机制，揭示并进一步调控脑缺血过程中外泌体miR-210的表达将有望成为缺血性脑损伤的治疗靶点，为进一步研究电针抗脑缺血再灌注损伤、发挥电针脑保护的机制提供新的思路和科学实验依据，从而指导针灸临床实践。

This project is based on our long term clinical and experimental research work about cerebral ischemia treated by electroacupuncture, combined with research progress on the relationships among exosome miRNAs and angiogenesis after cerebral ischemia at present. The project is to be studied both in vivo and in vitro the mechanism of exosome miR-210 to promote angiogenesis after cerebral ischemia treated by electroacupuncture therapy. Through the establishment of rat MCAO model and human umbilical vein endothelial cell (HUVEC) hypoxia model, making use of transmission electron microscope、laser confocal、Nanoparticle Tracking Analysis、immunohistochemistry、western blot、Real time PCR、siRNA、cell transfection and other techniques to analyze the relationship between electroacupuncture exosome miR-210、HIF-1、VEGF and Notch expression, through exosome miR-210 mediating HIF-1/VEGF/Notch signaling pathway to clarify regulation mechanism of angiogenesis after cerebral ischemia treated by electroacupuncture. Revealing and further regulating expression level of miR-210 in the course of cerebral ischemia will be a therapy target for ischemic brain injury. This project will provide scientific evidences for electroacupuncture against cerebral ischemia-reperfusion injury and brain protection, thereby instruct acupuncture clinical practice.