涡虫作为理想的再生和干细胞研究模式生物，具有大量的成体干细胞和强大的再生能力，Wnt信号是其再生中建立后端极性的关键信号通路，但分子机制尚未阐明。我们研究发现，TBX3基因与Wnt信号分子具有负相关的表达模式，敲低后，前端再生部位异常高表达Wnt信号，并形成尾部结构，产生前-后“双尾”的表型缺陷，提示TBX3可能通过负调控Wnt信号，介导涡虫再生中前-后轴极性的形成。在本项目中，我们拟以TBX3为切入点，研究TBX3对Wnt信号的具体调控途径，分析TBX3与Wnt信号在再生过程中对干细胞增殖与分化的影响，并进一步探讨它们在前-后轴极性形成过程中的作用模式，初步阐明TBX3负调控Wnt信号介导涡虫再生中前-后轴极性形成的机制，为干细胞和再生医学研究提供新的研究思路和有用信息。

The planarian is an ideal model organism to study stem cell and regeneration.It contains a high number of adult stem cells and owns powerful regenerative capacity. The Wnt signaling pathway plays an important role in the reformation of posterior polarity during the regeneration process. However, the underlying mechanism remains unclear. We found that the TBX3 gene showed a negatively correlated expression patterns with members of Wnt signaling. Knocked down of TBX3 induced the abnormally overexpression of Wnt signal at the anterior blastema, which caused the regeneration of head into the tail, resulting in a "double tail" phenotype defect in contrast with the phenotypes caused by down-regulate of Wnt signaling. These results suggest that TBX3 might control the formation of anterior-posterior axis polarity by negatively regulating the Wnt signaling. Based on the above information, this project aims to investigate the detailed regulation mechanism of TBX3 on Wnt signal, and analyze the effect of TBX3 and Wnt signaling on stem cell proliferation and differentiation. We will further explore the dynamic pattern of TBX3/Wnt signal during the formation of anterior-posterior axis polarity, and clarify the underlying mechanism of how TBX3 regulate the formation of anterior-posterior axis polarity by negatively regulating the Wnt signaling to provide important data basis for the research of stem cells and regenerative medicine.