镰刀菌是重要的单端孢霉烯族毒素产生菌。绿原酸是植物体内一类多酚类物质，可以显著抑制接骨木镰刀菌（Fusarium sambucinum）产T2毒素的积累。本项目采用微生物学方法研究经绿原酸处理对F. sambucinum菌落、孢子形态、孢子萌发、产孢量和生物量的影响，并借助UPLC-MS/MS测定T2毒素积累的变化；通过RT-PCR和转基因酵母表达载体等技术手段来分析T2毒素合成相关Tri基因表达及酶活的影响；采用iTRAQ定量蛋白质组学筛选经绿原酸处理前后F. sambucinum表达差异显著且与T2毒素合成相关的蛋白，并探究其生物学功能及在T2毒素合成途径的调控作用。以期阐明由绿原酸引发的F. sambucinumT2毒素合成调控机理，并寻找到有关单端孢霉烯族毒素合成途径新的调节因子。这些研究将对多酚类物质抑制单端孢霉烯族毒素的机理做出新的补充，为寻找到更有效的真菌毒素抑制剂提供参考。

Fusarium.spp is an important fungi producing trichothecenes. Chorogenic acid was a kind of polyphenols in plant, which significantly inhibited the accumulation of toxin T2 of Fusarium sambucinum. This research will explore the biological property of F. sambucinum after treated with chlorogenic acid, such as: the colony and spore morphology, spore germinstion, sporulation, and biomass. Simulaneously detect the accumulation of T2 toxic by the method of UPLC-MS/MS; Using RT-PCR, transgenic yeast expression vector to analysis of Tri gene expression and enzyme activity related to trichothecenes biosythesis; iTRAQ was used to quantitative proteomics to explore the expression differences of protein after treatment. All these explorations will elucidate the regulation mechanism of trichothecenes synthesis in F.sambucinum treated with chlorogenic acid and find new protein regulators. Finally all these works can complement the mechanism underlying the inhibiton of plant polyphenols to trichothecenes biosythesis, provide reference for searching more effective inhibitors of mycotoxins.