作为植物细胞中重要的磷脂酶之一，磷脂酶D (PLD) 可以水解膜骨架组分磷脂产生磷脂酸 (PA)，在脂类小分子参与的信号转导过程中起着关键的调控作用。近年来，越来越多的实验证据表明PLD和其产物PA在植物抵御病原微生物的侵染过程中具有启动抗病信号转导的功能。然而，PLD响应病原菌信号动态变化的调控机制尚不清楚。根据现阶段研究中存在的问题和不足，本研究着重开展下述三个方面的研究：1、研究拟南芥磷脂酶Dδ在病原菌入侵处的重定位机制；2、探讨细胞质膜以及膜微区在磷脂酶Dδ参与非寄主防御反应过程中的重要功能；3、分析磷脂酶Dδ重定位所依赖的转运途径在磷脂酶Dδ发挥抗病功能中的调控机制。本研究在高时空分辨率的水平上，通过细胞成像和量化分析，旨在揭示胞吐对于PLDδ响应病原菌信号的动态行为和重定位过程的调控机制，为研究胞吐在植物免疫反应过程中的调控机制提供新观点。

The phospholipase D (PLD) family of enzymes, which directly generate phosphatidic acid (PA) through the hydrolysis of structural phospholipids, plays a vital role in lipid based-signaling cascades in plants. Mounting evidence reported that PLD enzymes and PA were essential for activating multiple cellular responses in plant defense. Nevertheless, the spatiotemporal dynamics behaviors and underlying regulatory mechanism of PLD in response to pathogens remains to be fully elucidated. According to the problems and shortcomings in the current research, this study focuses on the following three aspects: 1. we will analyze the relocation mechanism of PLDδ at the pathogen invasion; 2. we will determine the partitioning of PLDδ within membrane microdomains under pathogen stimuli; 3. we will further explore the regulatory mechanism involving secretion in the relocation and resistant function of phospholipase Dδ. At the high temporal and spatial resolution level, this study aims to reveal the regulatory mechanism involving exocytosis in the relocalization and dynamic behaviors of PLDδ under pathogen stimuli, providing new insights into the regulation mechanism of exocytosis in plant immunity.