结核病是严重威胁人类生命健康的慢性、传染性疾病，特别是近年来耐药性的不断出现，使其死亡率更高，因此，急需开发新型有效的抗结核药物。海洋天然产物因其结构新颖、活性特殊已成为新药开发的重要宝库。基于二级质谱的分子网络技术能够快速排重已知化合物并提高新颖化合物的发现效率。在前期研究中，从一株柳珊瑚来源的海洋真菌Aspergillus versicolor 中发现了有抗结核活性的环肽化合物asperversiamides A–C，具有潜在的研究价值。在已有研究基础上，本项目拟在分子网络技术指导下，进一步挖掘该真菌产环肽化合物的结构多样性与新颖性，并对其进行抗结核活性评价和构效关系初步研究，以期发现结构新颖的抗结核活性化合物，为海洋新型抗结核先导物的筛选发现提供研究思路和化合物基础。

Tuberculosis is widespread, epidemic, infectious, and of high mortality. In recent years, drug-resistance forms of the disease have spread worldwide and become a global problem, and it is crucial to develope novel drugs to combat drug resistant strains of Mycobacterium. Marine natural products have proven to be a treasure with structurally novel and biologically active compounds that have become interesting and significant resources for drug discovery. The MS/MS-based molecular networking can be used to dereplicate known compounds and accelerate the discovery of new ones. Recently, three novel cyclopeptides, asperversiamides A–C, were isolated from the gorgonian-derived fungus Aspergillus versicolor. These compounds displayed anti-tubercular activities against different Mycobacterium species and had the potential for further research. This project will deeply explore the novelty and structural diversity of cyclopeptides from this fungus under the guidance of molecular networking, research on their anti-tubercular activities and structure-activity relationship, and discover novel anti-tubercular compounds. This project will provide research ideas and compound basis for screening and discovering new marine-derived anti-tubercular lead compounds.