亚微米色谱填料由于其高柱效而备受关注，但随着填料粒径的进一步减小，由于柱压过高限制了其在高效液相色谱中的应用。近年来纳米粒负载手性试剂以胶体溶液的形式作为准固定相在毛细管电泳中研究较多，且在手性分析中显著提高了色谱分离度。鉴于纳米粒负载手性试剂的高选择性，以及液液萃取为基础的高效离心分配色谱（HPCPC）与毛细管电泳在分离机理上均为液液现象的特征，提出制备亲脂性纳米硅胶粒键合手性试剂的胶体溶液应用于HPCPC手性分离中。研究内容：(1) 设计并制备亲脂性纳米硅胶颗粒键合手性试剂，表征微晶结构；(2) 探索毛细管电泳与手性液液萃取相结合的方法筛选HPCPC手性试剂；(3) 考察手性试剂微晶结构与手性拆分效果的相互关系；(4)HPCPC手性分离中溶质保留行为及相关理论。目标：建立以亲脂性纳米硅胶粒键合手性试剂为基础的HPCPC手性制备色谱新方法。

Recently the submicron chromatographic packing materials, such as submicron silica particles, have become very popular in separations by conventional liquid chromatography because of its high separation efficiency. However, much higher pressure of the separation column would be caused which is not suitable for conventional liquid chromatography if the packing materials with rather small particle size were used. A good case in point is the nanoparticles, such as gold nanoparticles and silica nanoparticles, which are very effective for enantioseparations if they are bonded or coated with chiral selectors, but they can not be applied in traditional liquid chromatography because of the high column pressure caused by the extremely tiny particle size. As a matter of fact, they have been mainly used as colloidal solution (pseudostationary phase) in capillary electrochromatography because no solid support is used for the stationary phase. .High performance centrifugal partition chromatography is a kind of preparative liquid-liquid partition chromatography using no solid support for the stationary phase, which is very similar with capillary electrochromatography with regarding to their separation mechanism. So far no report is available with regarding to preparation and application of nanoparticles bonded with chiral selectors in high performance centrifugal partition chromatography. Since a fundamental feature of capillary electorphoresis (a separation mechanism governed by solution phenomena involving no solid support) is shared by high performance centrifugal partition chromatography, the present program is aim to explore a new method for preparative enantioseparation by high performance centrifugal partition chromatography and to improve the separation efficiency for enantioseparations using the proposed colloidal solution as the chiral stationary phase for high performance centrifugal partition chromatography, in which the colloidal solutions are mainly composed of the nanoparticles bonded with chiral selectors. High enantiorecognition of chiral selectors are expected to be obtained because they are immobilized with the nanoparticles, which has also been proved in our tentative experiments. Meanwhile, a new method for screening the chiral selector used for liquid-liquid partition chromatography will be investigated by combination of high performance capillary electrophoresis and enantioselective liquid-liquid extraction techniques. The main topic of this project will be focused on the following four aspects: (1) Preparation and characterization of series of lipophilic nano particles supported chiral selectors; (2) To explore a method for selection of chiral selectors for high performance centrifugal partition chromatography by the combination of high performance capillary electrophoresis and enantioselective liquid-liquid extraction; (3) Application of the selected lipophilic nano particles bonded with chiral selectors in enantioseparation by high performance centrifugal partition chromatography, in which retention performance of the analytes along with equilibrium model will be studied; (4) The relationship between microcrystalline structure of nano particle supported chiral selectors and its enantiorecognition ability will be mainly investigated. The purpose of this project is to establish a novel preparative chiral separation method using high performance centrifugal partition chromatography with nano silica particle supported chiral selectors as chiral stationary phase.