近年来电活性生物材料对组织修复与功能重建的作用备受关注，但是临床转化策略一直难以突破。牙髓组织再生因其操作方便受周围环境影响小，一直被认为是组织工程研究的重要模型。针对目前牙髓干细胞（DPSCs）植入修复牙髓组织的过程中存在血管化不足、缺乏神经感受等问题，本项目提出设计构建三维仿生带电材料协同调控DPSCs多向功能分化促进全牙髓再生设计理念。结合前期基础，通过调节材料组分与极化参数，优化三维仿生电活性P(VDF-TrFE)/BaTiO3纳米复合材料性能，系统研究材料三维结构/力学/电学特性协同调控DPSCs成牙本质、成血管和成神经分化的作用，解析EphrinB2-EphB4等信号通路调节DPSCs多向功能分化的分子机制，最后通过动物实验验证电活性材料诱导后的DPSCs再生牙髓的有效性。本项目将为全牙髓组织再生修复提供有效策略，并拓展电活性材料在其它医学领域的应用策略。

In recent years, the application of electroactive biomaterials in tissue repair and functional reconstruction has attracted much attention, but clinical translation is difficult to achieve. Dental pulp regeneration is considered to be an important model in tissue engineering research because surgical procedures are easily and conveniently performed in a controlled setting, with little influence on the surrounding environment. To overcome insufficient angiogenesis and lack of neurological function during repair of pulp tissue by transplantation of dental pulp stem cell (DPSCs), this project puts forward the design concept of three-dimensional biomimetic electroactive materials to modulate the multi-directional functional differentiation of DPSCs to achieve whole pulp regeneration. Based on the foundation of our previous research data, the properties of the three-dimensional biomimetic electroactive p(VDF-TRFE)/BaTiO3 nanocomposites will be optimized by adjusting the material composition and polarization parameters. The effects of the three-dimensional structure/mechanical/electrical properties of the material on the co-regulation of odontogenic, angiogenic and neural differentiation of DPSCs will then be systematically studied. The molecular mechanisms of the multi-directional functional differentiation of DPSCs will be explored by analyzing EPHRINB2-EPHB4 and other signaling pathways. and finally the effectiveness of DPSCs (induced by the electroactive materials) in facilitating pulp regeneration will be evaluated by animal experiments. This project will provide an effective strategy for the regeneration and repair of whole pulp tissue, as well as expand the applications of electroactive materials to other biomedical fields.