抗性稗草（Echinochloa crus-galli）是严重威胁我国水稻安全生产的恶性杂草之一。前期研究发现GSTs介导稗草对噁唑酰草胺产生代谢抗性，但是介导稗草抗噁唑酰草胺的GST类型和功能，及GST介导的噁唑酰草胺在稗草体内代谢降解途径还不清楚。本项目拟在前期研究基础上，以药剂筛选和自然进化的抗噁唑酰草胺稗草生物型L4和WX15-RF1为研究对象，鉴定稗草对噁唑酰草胺代谢抗性相关GST基因，研究GST在抗性稗草体内的解毒功能，明确GST介导噁唑酰草胺在抗性稗草体内的代谢降解途径，从而阐明抗噁唑酰草胺稗草代谢抗性分子机制。预期研究结果有助于丰富GSTs介导的杂草抗药性机制理论，为发现新的除草剂分子靶标和研发新型除草剂以及培育抗除草剂转基因作物新品种提供依据，为抗性杂草的科学治理和水稻的安全生产提供支撑，应用前景广阔。

The resistant Echinochloa crus-galli is one of the harmful weeds that seriously threaten the rice production in China. Previous studies demonstrated that GSTs mediated metabolic resistance to metamifop in E. crus-galli. However, the structure and functional roles of the GST, and GST-mediated degradation pathways of metamifop in E. crus-galli is not yet clear. In this studies, low-dose herbicide selected and field evolved metamifop-resistant E. crus-galli biotypes L4 and WX15-RF1 used as the research material, the key GST genes associated with metabolism-based resistance will be identified, the functional roles of GST genes and GST-mediated degradation pathways of metamifop in E. crus-galli will be analyzed. Through these studies, the molecular mechanism of GST involved in metabolism-based metamifop resistance in E. crus-galli will be elucidated. The expected results will enrich the theoretical system of GSTs-mediated weed resistance mechanism and provide a basis for discovering new herbicide molecular targets, developing new herbicides and breeding new varieties of herbicide-resistant transgenic crops. Moreover, the research results will also provide support for the scientific management of resistant weeds and the safe production of rice, and have broad application prospects.