髓过氧化物酶（MPO）是生物体内与炎症直接相关的含血红素的过氧化物酶，其异常表达与多种疾病的发生发展关系密切。因此，在生物体系中对MPO进行识别和示踪，对于明确MPO与特定疾病的关系，及早采取诊疗措施，避免疾病的进一步发展恶化具有重要意义。但包括当前已经商用的MPO检测试剂盒在内，各种以荧光作为信号输出的MPO检测体系都是反应产物依赖型的，不能直接识别MPO，因而检测的精确度有限，并且不适用于对MPO的原位识别。针对这一问题，本项目拟选择萘酰亚胺作为荧光团，利用MPO抑制剂4-氨基苯甲酰肼（ABAH）作为识别基团，构建基于PET机理的非产物依赖型MPO有机小分子荧光探针。并利用所构建探针在体液、细胞、活体三个层次进行应用。本项目将首次尝试构建以荧光作为信号输出的非产物依赖型MPO荧光探针，以实现MPO的精确检测，为构建MPO体外检测试剂盒，在细胞、活体层次探索MPO的生理功能提供分子工具。

Myeloperoxidase (MPO) is a heme-containing peroxidase that is directly related to inflammation in living organisms and the abnormal expression of MPO is closely related to the development of various diseases. Therefore, the identification and tracing of MPO in biological systems is of great significance for clarifying the relationship between MPO and specific diseases, taking treatment measures to avoid further development of the disease. However, the reported various MPO detection methods based on fluorescence imaging (including the currently commercially available MPO fluorescence detection kits) are reaction product-dependent, which could not identify MPO directly and is not suitable for MPO in-situ identification.To solve this problem, we intend to select naphthimide as the fluorophore and use MPO inhibitor 4-aminobenzohydrazide (ABAH) as the recognition group to construct product-independent MPO organic small molecule fluorescent probes based on PET mechanism in this work. And the probes constructed will be applied in vitro and in vivo. The development of this project will fill the current gaps in the absence of product-independent MPO fluorescent probes, and provide new tools for constructing MPO in vitro detection kits, exploring the physiological functions of MPO in vitro and in vivo.