半月板是膝关节的重要结构，一旦损伤会影响患者膝关节功能和身心健康。组织工程半月板（TEM）的研究领域主要集中在细胞、支架、细胞因子等方面，却忽略了TEM在体外培育或植入体内后会遭受炎症和氧化应激等复杂再生微环境的影响。迄今为止，所培育TEM的外观形貌、组织结构、力学强度及功能应用均不能满足临床需求，这和TEM在体内外遭受的炎症和氧化微环境有一定关系。本项目拟结合个性化3D打印、药物控释和Ac2-26纳米颗粒抗炎抗氧化调节微环境等协同作用，制备并筛选得到具有异质性仿生结构、优异生物力学性能和抗炎抗氧化功效的多功能半月板双相支架。通过调节TEM体内外的抗炎抗氧化微环境，分析TEM的细胞活性和分化、组织学、生物力学、影像学、多组学等结果，探讨抗炎抗氧化微环境对结构和功能异质性TEM的构建、生长进程调控和关节软骨保护的作用效果，研究TEM质量提升与否的机制，以期培育出高质量的TEM并应用于临床。

The meniscus is an important structure of the knee joint. Once the meniscus is damaged, it will affect the knee function and physical and mental health of the patients. The research field of tissue engineering meniscus (TEM) mainly focuses on cells, scaffolds, cytokines and so on, but ignores that TEM will be affected by complex regeneration microenvironments such as inflammation and oxidative stress after in vitro cultivation or implantation in vivo. So far, the morphology, tissue structure, mechanical strength, and functional applications of the cultivated TEMs have not been able to meet clinical needs and have a certain relationship with the microenvironment of TEMs that suffer inflammation and oxidation in vitro and in vivo. The study intends to combine personalized 3D printing technology, controlled drug release methods and Ac2-26 nanoparticles anti-inflammatory and anti-oxidant regulation microenvironments to prepare and screen versatile meniscus biphasic scaffold with heterogeneous bionic structure, excellent biomechanical properties and anti-inflammatory and anti-oxidant effects. By adjusting the anti-inflammatory and anti-oxidation microenvironment of TEM in vitro and in vivo, we analyze the results of cell activity and differentiation, histology, biomechanics, imaging and multi-omics analysis of TEM. We also explored the effects of anti-inflammatory and anti-oxidant microenvironments on construction of structural and functional heterogeneous TEM, growth process regulation and articular cartilage protection, and studied the mechanism of whether the TEM quality is improved in this process, with a view to cultivating a high-quality of TEM and applying it to the clinic.