除草剂百草枯慢性低剂量暴露诱导的中枢神经系统损伤是目前国内外的研究热点，免疫反应介导的神经炎症过程是百草枯诱导神经损伤和发育障碍的重要环节，而细胞因子信号通路在神经炎症的发生发展过程中起重要调控作用，但具体机制尚不明确。因此，本项目拟采用整体动物和离体神经干细胞模型，以免疫细胞的活化种类和浸润程度以及细胞因子信号通路中的关键分子作为检测靶点，结合高通量芯片及生物信息学技术，探讨神经炎症的发生发展对于百草枯所致中枢神经系统损伤及发育障碍的影响，并阐明细胞因子相关信号通路在其中的调控机制。项目的实施可以为百草枯诱导的中枢神经系统损伤提供免疫学证据，进一步完善百草枯的神经毒性机制理论，从而为百草枯的管理和干预提供科学依据。

Paraquat (PQ) is a world-widely used herbicide compound and the impact of low dose exposure to PQ on central nervous system (CNS) has been extensively studied. The neuroinflammation mediated by immune response is an important intermediate element for the successive neural system damage and/or neurodevelopmental disorders, and the cytokine mediated signaling pathways play a vital role in the progression of neuroinflammation, however the mechanism in detail is not fully clarified. Thus in the current project we aim to assess the activation and infiltration of immune cells as well as the expression of cytokines related signaling molecules by using mice model in vivo and neural stem cells in vitro, combined with intervention trial of relevant drugs, to explicit the role of neuroinflammation in the neurotoxicity induced by PQ. This work will provide immunological evidence for neurotoxical mechanism induced by PQ, which will contribute to the PQ management and intervention.