本研究针对华山松大小蠹对寄主华山松入侵的单一性和种群密度相对稳定性，特别是华山松大小蠹入侵和危害过程中神经肽NPF基因对营养积累、卵发育的调控作用研究的空白，在系统研究华山松大小蠹种群动态、寄主选择、消化道结构和精子发生等基础上，应用功能基因组学、生物化学和分子生物学等技术，通过对华山松大小蠹不同生理状态NPF基因转录组分析，华山松大小蠹特定NPF的鉴定、合成与免疫组织定位，华山松大小蠹特定NPF基因表达水平与取食、营养积累的关系，华山松大小蠹特定NPF对卵巢发育主要调控机制的研究，揭示神经肽NPF基因对华山松大小蠹营养积累和卵发育的调控作用，具有重大的科学意义和理论研究价值，将对小蠹类森林害虫的种群繁衍、寄主选择和种群密度调控提供科学依据。

The Chinese white pine beetles specifically invade the Chinese white pine and population density relative stability in the Qinling Mountain, little is known especially about neuropeptide F regulation on nutrient accumulation and ovarian development during the process of Chinese white pine beetle invasion and destruction. Based on the population dynamics, host selection, structure of the digestive tract and spermatogenesis of Chinese white pine beetle, the research project will adopt the functional genomics, biochemistry and molecular biology technologies, to analyze Chinese white pine beetle transcriptomics under different physiological states, Chinese white pine beetle NPF identification, synthesis and immunohistochemical localization, relationship between specific NPF gene expressions and nutrient accumulation and ovarian development and regulation mechanism of specific NPF on Chinese white pine beetle ovarian development. The regulatory functions of neuropeptides F in nutrient accumulation and have scientific significance and theoretical vales for explaining the population regulation and host selection of Chinese white pine beetles, and providing the scientific basis for the bark beetles pest management.