Functional Characterization of Allatostatin C (PISCF/AST) and Juvenile Hormone Acid O-Methyltransferase in Dendroctonus armandi.
Functional Characterization of Allatostatin C (PISCF/AST) and Juvenile Hormone Acid O-Methyltransferase in Dendroctonus armandi.
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DOI：
10.3390/ijms23052749
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发表时间：
2022-03-02
影响因子：
5.6
通讯作者：
Chen H
中科院分区：
文献类型：
--
作者：
Sun Y;Fu D;Liu B;Wang L;Chen H
Allatostatin C （PISCF/AST）是一种影响幼崽激素（JH）合成的神经肽基因。幼激素酸o -甲基转移酶（JHAMT）是JH生物合成途径的关键基因。本研究克隆了两个编码DaAST和DaJHAMT的基因。DaAST和DaJHAMT在中华白松甲虫幼虫、蛹和成虫中均有表达，且在头部和肠道中均有高表达。用JH类似物（JHA）甲基戊二烯诱导这两个基因的表达，并利用RNAi技术研究这两个基因的功能。考虑到激素在变态中的作用，JHA在幼虫期显著诱导DaAST和DaJHAMT。在幼虫、蛹和成虫中，DaJHAMT mRNA水平显著升高。此外，敲低DaAST而不是DaJHAMT会增加蛹死亡率和羽化形态异常率，降低羽化率。然而，敲除DaJHAMT而不是DaAST显著降低了成年雄性额叶蛋白的生物合成。结果表明，DaAST具有抑素作用，可抑制JH的合成，而JHAMT是曼陀罗合成JH的关键调控酶。
Allatostatin C (PISCF/AST) is a neuropeptide gene that affects juvenile hormone (JH) synthesis in the corpora allata. Juvenile hormone acid O-methyltransferase (JHAMT) is a key gene in the JH biosynthetic pathway. In this study, two genes encoding DaAST and DaJHAMT were cloned. Both DaAST and DaJHAMT were expressed in the larvae, pupae and adults of Chinese white pine beetle (Dendroctonus armandi), and highly expressed in the head and the gut. The expression of the two genes was induced by JH analog (JHA) methoprene and the functions of the two genes were then investigated by RNAi. Considering the role of hormones in metamorphosis, JHA significantly induced DaAST and DaJHAMT in the larval stage. DaAST knockdown in larvae, pupae and adults significantly increased the DaJHAMT mRNA levels. Moreover, knockdown of DaAST instead of DaJHAMT increased pupae mortality and the abnormal rate of emergence morphology and reduced emergence rates. However, knockdown of DaJHAMT instead of DaAST significantly reduced frontalin biosynthesis in adult males. The results showed that DaAST acts as an allatostatin and inhibits JH biosynthesis, and that JHAMT is a key regulatory enzyme for JH synthesis in the D. armandi.
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影响因子：
2.2
作者：
Blackburn, MB;Jaffe, H;Raina, AK
通讯作者：
Raina, AK
影响因子：
4.6
作者：
Borras-Castells F;Nieva C;Maestro JL;Maestro O;Belles X;Martín D
通讯作者：
Martín D
影响因子：
--
作者：
BELLES, X;MAESTRO, JL;THORPE, A
通讯作者：
THORPE, A
DOI：
10.1073/pnas.1219733110
发表时间：
2013-01-08
影响因子：
11.1
作者：
Bhargava, Aditi;Clifton, Matthew S.;Grady, Eileen F.
通讯作者：
Grady, Eileen F.
影响因子：
3.8
作者：
Dai, Lulu;Ma, Mingyuan;Chen, Hui
通讯作者：
Chen, Hui