黑芥子酶-特异蛋白互作精细调控芥子油苷代谢的机制在植物应对各种生物和非生物胁迫的防卫反应中至关重要。研究表明特异蛋白与黑芥子酶的互作需要辅因子参与，但迄今为止尚无相关辅因子的报道。AKR2A是拟南芥中新发现的分子伴侣。我们研究发现AKR2A与黑芥子酶TGG1和TGG2在植物体内体外互作。akr2a突变体中黑芥子酶蛋白水平及定位均受抑制。经蛋白质组分析，akr2a突变体中特异蛋白ESP和ESM1水平显著下降而且AKR2A与二者在酵母中互作。基于此，本项目拟分三个步骤对AKR2A在黑芥子酶-特异蛋白互作中的作用机制进行研究：①解析AKR2A与黑芥子酶互作的分子机制；②解析AKR2A与特异蛋白互作的分子机制；③探索AKR2A在黑芥子酶-特异蛋白互作中的必要性及三者的互作模型。关于伴侣分子参与调控芥子油苷代谢的研究鲜有报道，本项目的研究将丰富芥子油苷代谢通路，推进芥子油苷代谢调控网络研究的进程。

The mechanism of myrosinase and specifier proteins interacting to control and fine-tune glucosinolates breakdown appears to be essential for appropriate plant responses to various biotic as well as abiotic stresses. According to previous studies, cofactors are required for the interaction between myrosinase and specifier proteins. However, there have no such cofactors been reported until now. AKR2A was a newly found molecular chaperone in Arabidopsis.In our previous research, we found that TGG1 and TGG2 were interacted with AKR2A no matter in vitro or in vivo.The expression level and location of TGG1 and TGG2 were decreased statistically in akr2a mutants. Besides, based on iTRAQ analysis of akr2a-1, the expression level of specifier proteins ESP and ESM1 was also decreased. The H2Y analysis demonstrated that AKR2A also interacted with ESP and ESM1. Based on these results, we plan to reveal the action mechanism of AKR2A on myrosinase and specifier proteins interaction by three steps: ① studying the interaction mechanism among AKR2A, TGG1 and TGG2 by founding out how AKR2A interacted with TGG1 and TGG2 and affected their gene transcription, protein expression and location; ② investigating the interaction mechanism among AKR2A, ESP and ESM1; ③ studying the regulation mechanism of AKR2A on the interaction of myrosinase and specifier proteins by verifying the necessity of AKR2A and the interaction model of these three. The results will reveal more elements in glucosinolates metabolic pathway and push forward our understandings of glucosinolates metabolic regulatory network.