三阴乳腺癌（TNBC）具有侵袭转移快、复发性强的特点，是目前临床治疗的难点。DNA损伤应答系统可以作为治疗TNBC有效的靶点，本研究拟利用侵袭转移过程中的DNA损伤应答通路来寻找可能的治疗靶点。前期通过TNBC临床样本的组学数据分析发现PARP1（多聚二磷酸腺苷核糖合成酶1）和XRCC1（X射线损伤修复的交叉互补蛋白1）均表达上调，免疫共沉淀表明PARP1和XRCC1存在相互作用。因此，拟以三阴乳腺癌细胞和荷瘤动物为研究平台，先阐明PARP1与XRCC1的相互作用关系，在DNA损伤修复中发挥的作用和调控TNBC侵袭转移的分子机制。最后在临床水平上，进一步研究XRCC1和PARP1的表达水平及调控TNBC侵袭转移的作用机制，同时对TNBC侵袭转移和患者数据进行相关性分析。本课题聚焦于PARP1和XRCC1，从新的角度解析和阐明TNBC侵袭转移的作用机制，预期结果将为治疗TNBC提供新的靶点。

Three-negative breast cancer (TNBC) has the characteristics of rapid invasion and metastasis, and it is the current difficulty in clinical treatment. Genes that utilize the DNA damage response pathway during TNBC invasion and metastasis are effective strategy for characterizing therapeutic targets. In the preliminary work, we found that PARP1 and XRCC1 function as complex, as well as up-regulated in TNBC. Therefore, this study aims to elucidate the interaction between PARP1 and XRCC1 by starting with the function and mechanism of PARP1 and the interaction protein XRCC1 in the DNA damage response pathway. To regulate the mechanism of DNA damage repair of TNBC, and further analyze the relationship between the expression level of XRCC1 and PARP1 in TNBC and TNBC invasion and metastasis, in order to inhibit the invasion and metastasis of TNBC. And treatment offers new strategies. This topic will provide new ideas for the treatment of TNBC invasion and metastasis by using the functional proteins PARP1 and XRCC1 of the DNA damage response pathway, thus providing a new target for clinical treatment.