西瓜连作土传枯萎病严重制约着我国西瓜产业的可持续发展。土壤绿色熏蒸联合生物有机肥能够有效防控西瓜土传枯萎病，促进西瓜生长，但是其促生防病的机理还不清楚。本项目以优良的田间试验效果为基础提出科学问题，从土壤棉隆熏蒸这一环境友好型的土壤处理方法入手，利用胞外DNA去除，荧光标记，定量PCR，高通量测序等技术手段辅以生物信息分析方法，分析西瓜枯萎病高发病土壤经熏蒸处理后土壤残留活性微生物特征及其恢复过程，再通过接种植物益生菌修复发病土壤，研究残留活性微生物对益生菌定殖的影响，同时利用盆栽试验评估该方法在四种不同类型土壤上修复微生物区系的效果以及防病促生效果的持久性与普适性。通过项目的实施，阐明土壤绿色熏蒸联合生物有机肥修复西瓜枯萎病高发病土壤微生物区系的机制，实现用含大量植物益生菌的生物有机肥配施来定向培育熏蒸土壤的微生物区系，使土壤恢复健康，达到培育高产抑病型土壤的目的。

Fusarium wilt of watermelon caused by continuous mono-cropping seriously restricts domestic watermelon production. Soil fumigation coupled with bioorganic fertilizer application succeeded in suppressing Fusarium wilt in watermelon cropping, however the mechanisms in disease control and plant growth promotion is still not clear. Based on the results from the field experiment, we propose to study 1) the characteristics of the live microbial community after fumigation and its recover process, 2) how these live microorganisms influence the beneficial strain colonization, and 3)the duration and stability of the rebuilt microbial community as well as the disease control effect on the four typical soils through external DNA removal, GFP-tagging, realtime PCR, and high throughput sequencing. Base on the results, we will reveal how to restore the microbiome in the soil with severe Fusarium wilt through soil fumigation and bio-organic fertilizer application. The results will provide fundamental basis for disease suppressive soil management through soil microbiome transplant as a result to suppress soil-borne disease.