胃癌是我国发病率及死亡率占第二位的恶性肿瘤。虽然对胃癌的科学研究和临床诊治取得了一定进展，但胃癌发病率仍然居高不下。当今，科学家们更加关注针对干细胞是否能够找到特异性生物标志物miRNA。本课题深入地以miR-1915、miR-4308为靶点开展研究。提出科学假说：（1）生物活性肽ACBP可能通过靶向调节miRNA影响胃癌干细胞生物学行为，下调Bcl-2的表达；（2）miRNA-1915、miRNA-4308/ Bcl-2可能组成调控网络，抑制胃癌干细胞增殖。项目拟从分子、细胞、动物水平以及临床肿瘤标本四个层次验证假说，深入阐明ACBP介导miRNA对分化不同的胃癌干细胞增殖、凋亡、侵袭的分子机制，探索ACBP-miRNA-干细胞-靶基因网络调控与胃癌治疗的相关机制，证实miRNA1915、miRNA4308的抗肿瘤功能，为以miRNA为靶标的胃癌生物治疗提供科学的理论与实验数据。

Gastric cancer is the second malignant tumor in the incidence and mortality of our country. Although some progress has been made in the scientific research and clinical diagnosis of gastric cancer, the incidence is still high. Nowadays, scientists are more concerned with stem cells are able to find the specific biomarkers miRNA. we take miR-1915 and miR-4308 as the target to carry out these research. To put forward the scientific hypothesis: (1) bioactive peptide ACBP may be regulated miR-1915 and miR-4308 by targeting to influence the biological behavior of gastric cancer stem cells, down regulation of Bcl-2 expression; (2) miRNA-1915 and miRNA-4308/Bcl-2 are compose to the regulatory network possibly and to restrain the proliferation of gastric cancer stem cells. The hypothesis verification work will be done from the four levels including molecular level, cell level, animal level and clinical specimens . To further elucidation that ACBP mediated microRNA could influence on the mechanism of proliferation, apoptosis and invasion of gastric cancer stem cells in different differentiation, to explore the relevant mechanism of regulation of ACBP-microRNA-stem cell target gene network and gastric cancer therapy, to confirme the anti-tumor function of miRNA1915 and miRNA4308, to provide scientific theory and experimental data for biological therapy of gastric cancer with miRNA as target.