联合用药是肿瘤治疗中降低毒副作用和增强化疗效果的重要手段之一。前期研究发现，奥沙利铂联合生物活性肽（BAP）不但可以明显降低奥沙利铂的使用量，而且减轻副作用，但是具体分子调控机理尚未阐明。预实验结果显示联合作用与TPX2的表达下调相关。TPX2与胃癌发生和发展密切相关，是抗癌药物研发的重要靶点之一。据此提出科学假设：BAP协同奥沙利铂治疗胃癌可起到减毒增敏作用；分子机理可能涉及TPX2的表达下调，从而影响了所介导的PI3/AKT信号通路，影响了下游靶分子Aurora A以及细胞周期进程，最终抑制胃癌的发生与发展。项目以临床样本、细胞实验、动物体内实验和生物信息学分析等方法解释BAP联合奥沙利铂减毒增敏作用，阐明联合用药调控TPX2蛋白表达从而抑制胃癌发生发展的分子机制，揭示BAP联合奥沙利铂在胃癌治疗过程中潜在应用价值，为胃癌靶向联合治疗提供新思路。

The drug combination is one of the excellent treatment methods to reduce toxic side effects and provide a positive impact on the effect of chemotherapy. Our previous study has found that oxaliplatin combined with bioactive peptide (BAP) is not only significantly reduce the consumption of oxaliplatin but also minimise side effects. However, the molecular mechanism is still unclear. Pre-experimental results suggest that this effect may be associated with the down-regulation of TPX2 expression. TPX2 is one of the critical targets for the development of anticancer drugs due to is closely related to the occurrence and development of gastric cancer. We proposed a hypothesis that BAP combined with oxaliplatin in the treatment of gastric cancer can play a role in the toxicity reduction and improving the therapeutic efficiency of oxaliplatin;The molecular mechanism may be down-regulated by the expression of TPX2, which affects the mediated PI3/AKT signaling pathway, the downstream target molecule Aurora A and cell cycle progression, and finally suppresses the occurrence and development of gastric cancer. This research combined the use of clinical samples, cell experiments, in vivo experiments in animals and bioinformatics analysis to explain the molecular mechanism of BAP combined with oxaliplatin for toxicity reduction and therapeutic efficiency improvement. In particular, it was demonstrated that this drug combination method down-regulated TPX2 protein expression and thereby inhibits the development of gastric cancer. The potential application value of BAP combined with oxaliplatin in the treatment of gastric cancer was clarified, which provided a promising idea for targeted therapy of gastric cancer.