骨质疏松状态会严重影响口腔的正畸治疗。桃叶珊瑚苷从杜仲中获得的单体，具有抗氧化等活性。本课题组率先报道了桃叶珊瑚苷促成骨细胞分化的作用，基于此前期基础，本项目将研究桃叶珊瑚苷对骨质疏松状态下正畸牙移动过程中成骨细胞和破骨细胞分化、凋亡及功能的影响；建立骨质疏松小鼠正畸牙移动模型，测定血液及骨组织中成骨及破骨标志物蛋白表达、矿物质含量、矿物沉积速率、骨组织显微结构及骨密度等的变化，证实桃叶珊瑚苷治疗骨质疏松的作用和在骨质疏松状态下对正畸牙移动的影响；项目将率先将蛋白质组学同代谢组学结合，系统筛选桃叶珊瑚苷在骨质疏松治疗中的潜在蛋白靶点，并对药物同靶蛋白的分子对接进行模拟。本项目的实施将为天然化合物治疗骨质疏松的研究提供思路，为桃叶珊瑚苷作为骨质疏松状态下正畸牙移动的治疗药物提供系统的药理学基础。

The state of osteoporosis will seriously affect orthodontic treatment.Aucubin, a kind of iridoid glycosides mainly obtained from Eucommiaulmoides, possesses antioxidant, liver protective and anti-inflammatory activities. Our group has successfully found that aucubin improved osteoblast differentiation. Based on our experimental foundations, the present project will firstly investigate the effects of aucubin on the differentiation, apoptosis and function of osteoblastand osteoclastcells within osteoporosis status and orthodontic teeth movement mechanisms related to oxidative stress-mediated Smads signaling. Furthermore, the anti-osteoporosis effects of aucubin will be investigated in mouse models with osteoporosis and orthodontic tooth movement. The expressions of osteogenic and osteoclastic markers protein, mineral content, mineral deposition rate, bone tissue microstructure and bone mineral density in blood and bone of mice will be analyzed. Additionally, in this project, we will combine the proteome and metabonomics together, which will be applied to screen the protein targets during aucubin-mediated anti-osteoporosis in the mouse model. This project will provide ideas for natural compounds in the treatment of osteoporosis and provide a systematic pharmacological basis for aucubin as a therapeutic drug for osteoporosis and orthodontic teeth movement in osteoporosis.